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pCAL-kc

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At a Glance

pCAL-kc From Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter

lac Operator

MCS

lacI repressor ORF

Accession Number




Notes

Express affinity tagged fusion protein in bacteria for enrichment of Calmodulin based resin.

History

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Sources

Agilent-Genomics

References

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Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GCTAGCATGA  
 101  CTGGTGGACA GCAAATGGGT CGGATCCCTT AGACGCGCAT CACTTGGTAG  
 151  ATCCATGTAT CCACGTGGGA ATGGTACCAA GCGACGATGG AAAAAGAATT  
 201  TCATAGCCGT CTCAGCAGCC AACCGCTTTA AGAAAATCTC ATCCTCCGGG  
 251  GCACTTTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG  
 301  CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG  
 351  GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCCGC  
 401  AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG  
 451  GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG  
 501  GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC  
 551  TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC  
 601  TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT  
 651  TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG  
 701  TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC  
 751  CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA  
 801  CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT  
 851  TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT  
 901  TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC  
 951  CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA  
1001  AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC  
1051  AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA  
1101  CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG  
1151  CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA  
1201  CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG  
1251  GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT  
1301  ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT  
1351  TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA  
1401  TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC  
1451  GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC  
1501  GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC  
1551  CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG  
1601  AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC  
1651  TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT  
1701  TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC  
1751  CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG  
1801  AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC  
1851  TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA  
1901  TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC  
1951  AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC  
2001  AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC  
2051  AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA  
2101  GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG  
2151  TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT  
2201  ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG  
2251  ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG  
2301  CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA  
2351  CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC  
2401  TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC  
2451  TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA  
2501  TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA  
2551  CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG  
2601  CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG  
2651  TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC  
2701  ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC  
2751  AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT  
2801  ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA  
2851  CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG  
2901  GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT  
2951  TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG  
3001  TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG  
3051  ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA  
3101  CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG  
3151  GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA  
3201  GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA  
3251  GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT  
3301  GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA  
3351  TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC  
3401  GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG  
3451  CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA  
3501  GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG  
3551  ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC  
3601  CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG  
3651  GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG  
3701  ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG  
3751  CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC  
3801  AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG  
3851  AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA  
3901  ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG  
3951  GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC  
4001  GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG  
4051  CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC  
4101  GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC  
4151  GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA  
4201  AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG  
4251  AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GAGATCCCGG  
4301  TGCCTAATGA GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG  
4351  CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA  
4401  CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCCAGGGTG GTTTTTCTTT  
4451  TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC CTGGCCCTGA  
4501  GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC  
4551  CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT  
4601  CGTCGTATCC CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG  
4651  GTAATGGCGC GCATTGCGCC CAGCGCCATC TGATCGTTGG CAACCAGCAT  
4701  CGCAGTGGGA ACGATGCCCT CATTCAGCAT TTGCATGGTT TGTTGAAAAC  
4751  CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG CTGAATTTGA  
4801  TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC  
4851  AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA  
4901  CCAGATGCTC CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA  
4951  CTGTTGATGG GTGTCTGGTC AGAGACATCA AGAAATAACG CCGGAACATT  
5001  AGTGCAGGCA GCTTCCACAG CAATGGCATC CTGGTCATCC AGCGGATAGT  
5051  TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG CACCGCCGCT  
5101  TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC  
5151  ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG  
5201  CGTGCAGGGC CAGACTGGAG GTGGCAACGC CAATCAGCAA CGACTGTTTG  
5251  CCCGCCAGTT GTTGTGCCAC GCGGTTGGGA ATGTAATTCA GCTCCGCCAT  
5301  CGCCGCTTCC ACTTTTTCCC GCGTTTTCGC AGAAACGTGG CTGGCCTGGT  
5351  TCACCACGCG GGAAACGGTC TGATAAGAGA CACCGGCATA CTCTGCGACA  
5401  TCGTATAACG TTACTGGTTT CACATTCACC ACCCTGAATT GACTCTCTTC  
5451  CGGGCGCTAT CATGCCATAC CGCGAAAGGT TTTGCGCCAT TCGATGGTGT  
5501  CCGGGATCTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC  
5551  CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT  
5601  GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA  
5651  CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC  
5701  CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC  
5751  CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC  
5801  CCGCGAAAT