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pCAL-n-EK

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At a Glance

pCAL-n-EK, Image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

T7 Promoter
T7 Gene 10 RBS
Calmodulin-binding peptide
EK target
Thrombin target
Multiple cloning site
lacI repressor

Accession Number

GenBank: U86347.1



Notes

Expresses affinity tagged fusion proteins in bacteria for enrichment by a Calmodulin based resin. This plasmid contains T7 promoter and terminator, multiple cloning sites, EK and thrombin targets, pBR322 origin, and ampicillin resistance. It also contains a calmodulin-binding peptide.

History

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Sources

Stratagene

References

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Sequence

THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO OCTOBER, 2001.

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GAAGCGACGA  
 101  TGGAAAAAGA ATTTCATAGC CGTCTCAGCA GCCAACCGCT TTAAGAAAAT  
 151  CTCATCCTCC GGGGCACTTC TGGTTCCGCG TGGATCTGGT TCTGGTGATG  
 201  ACGACGACAA GGGAAGAGGA TCCGAATTCT CTTCCCGGGT CTTGTTCCAT  
 251  GGGTCGACTC GAGCTCAAGC TTAGATCCGG CTGCTAACAA AGCCCGAAAG  
 301  GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT  
 351  TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA  
 401  TATCCGGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA  
 451  TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG  
 501  TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA  
 551  ACTACCGCAT TAAAGCTAAT CGAAGATAAG CTGTCAAACA TGACAATTCT  
 601  TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT  
 651  GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC  
 701  GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG  
 751  CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA  
 801  CAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG  
 851  CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA  
 901  GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT  
 951  CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA  
1001  TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT  
1051  GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA  
1101  CTTGGTTGAC GCGTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA  
1151  CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG  
1201  GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT  
1251  TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG  
1301  AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA  
1351  GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT  
1401  AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG  
1451  GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA  
1501  TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC  
1551  AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG  
1601  CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG  
1651  ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT  
1701  TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT  
1751  TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA  
1801  GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT  
1851  TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG  
1901  TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT  
1951  GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA  
2001  GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC  
2051  TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT  
2101  ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG  
2151  CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA  
2201  CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC  
2251  GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG  
2301  AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC  
2351  CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG  
2401  TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG  
2451  GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT  
2501  CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC  
2551  GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC  
2601  GGAAGACCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT  
2651  CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT  
2701  AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG  
2751  CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG  
2801  CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT  
2851  GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT  
2901  AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA  
2951  TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT  
3001  GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG  
3051  CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA  
3101  CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT  
3151  ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC  
3201  AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA  
3251  GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT  
3301  AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA  
3351  AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG  
3401  CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT  
3451  AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA  
3501  TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG  
3551  CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG  
3601  CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG  
3651  TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC  
3701  GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG  
3751  GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA  
3801  AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG  
3851  CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG  
3901  GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG  
3951  AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA  
4001  CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG  
4051  CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG  
4101  CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC  
4151  GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT  
4201  ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT  
4251  GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA  
4301  TCGGTCGAGA TCCCGGTGCC TAATGAGTGA GCTAACTTAC ATTAATTGCG  
4351  TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA  
4401  TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC  
4451  AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT  
4501  CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC  
4551  CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG GATATAACAT  
4601  GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATAT CCGCACCAAC  
4651  GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT  
4701  CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC  
4751  ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC  
4801  TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC  
4851  GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG CGCGATTTGC  
4901  TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG TACCGTCTTC  
4951  ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG ACATCAAGAA  
5001  ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG  
5051  TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG  
5101  ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG  
5151  ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT AATCGCCGCG  
5201  ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG CAACGCCAAT  
5251  CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG TTGGGAATGT  
5301  AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT TTTCGCAGAA  
5351  ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT AAGAGACACC  
5401  GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCACA TTCACCACCC  
5451  TGAATTGACT GTCTTCCGGG CGCTATCATG CCATACCGCG AAAGGTTTTG  
5501  CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA TGCGACTCCT  
5551  GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC  
5601  GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC  
5651  GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT  
5701  GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA  
5751  ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG  
5801  GATCGAGATC TCGATCCCGC GAAAT