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pET-11a

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At a Glance

pET-11a, Image provided by Novagen

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
Ribosome binding site
Nde I cloning site
BamHI cloning site
lacI repressor
pBR322 origin

Accession Number




Notes

In the pET system the sequence of interest is transformed into E. coli after being transcribed downstream from the T7 promoter and the gene 10 ribosome binding site. One can use IPTG induction of a chromosomally integrated cassette or infection with a polymerase-expressing bacteriophage lamba CE6 to achieve protien expression. After induction to E. coli the very active polymerase cause a high nimber of transcipts. Due to this phenomenon and high efficiency of translation, the target protein may become the majority of the cellular protein in only a matter of hours. [1]

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent Genomics

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

  1. Bonin, C. "PNAS,Vol 94". 1996. Page 2085-9090.

Sequence

THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGCTAGCATG  
 101  ACTGGTGGAC AGCAAATGGG TCGCGGATCC GGCTGCTAAC AAAGCCCGAA  
 151  AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC  
 201  CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC  
 251  TATATCCGGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC  
 301  TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT  
 351  TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT  
 401  AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT  
 451  CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC  
 501  ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT   
 551  GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC  
 601  CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG  
 651  AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC  
 701  GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA  
 751  AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT  
 801  CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC  
 851  AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG  
 901  TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT  
 951  GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT  
1001  GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG  
1051  CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT  
1101  TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC  
1151  GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG  
1201  CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT  
1251  CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC  
1301  AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA  
1351  AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG  
1401  CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA  
1451  GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC  
1501  TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG  
1551  ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT  
1601  TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT  
1651  GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT  
1701  TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC  
1751  GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA  
1801  CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG  
1851  TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC  
1901  TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC  
1951  TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG  
2001  GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA  
2051  CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC  
2101  CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA  
2151  GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG  
2201  TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT  
2251  CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA  
2301  CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT  
2351  ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA  
2401  CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA  
2451  GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT  
2501  TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC  
2551  ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC  
2601  TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC  
2651  TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC  
2701  ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG  
2751  GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT  
2801  CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT  
2851  CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA  
2901  TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA  
2951  AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG  
3001  TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA  
3051  CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG  
3101  TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC  
3151  ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG  
3201  GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC  
3251  AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA  
3301  GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT  
3351  CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC  
3401  GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT  
3451  TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT  
3501  GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC  
3551  CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG  
3601  CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA  
3651  TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG  
3701  AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC  
3751  TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA  
3801  AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA  
3851  GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT  
3901  CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG  
3951  TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA  
4001  GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC  
4051  CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC  
4101  ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG  
4151  CATCGGTCGA GATCCCGGTG CCTAATGAGT GAGCTAACTT ACATTAATTG  
4201  CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG  
4251  CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG  
4301  CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC  
4351  TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG  
4401  CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC  
4451  ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT ATCCGCACCA  
4501  ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG  
4551  ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT  
4601  GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC  
4651  GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG  
4701  ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT  
4751  GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT  
4801  TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG AGACATCAAG  
4851  AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT  
4901  GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA  
4951  AGATTGTGCA CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT  
5001  CGACACCACC ACGCTGGCAC CCAGTTGATC GGCGCGAGAT TTAATCGCCG  
5051  CGACAATTTG CGACGGCGCG TGCAGGGCCA GACTGGAGGT GGCAACGCCA  
5101  ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC GGTTGGGAAT  
5151  GTAATTCAGC TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG  
5201  AAACGTGGCT GGCCTGGTTC ACCACGCGGG AAACGGTCTG ATAAGAGACA  
5251  CCGGCATACT CTGCGACATC GTATAACGTT ACTGGTTTCA CATTCACCAC  
5301  CCTGAATTGA CTCTCTTCCG GGCGCTATCA TGCCATACCG CGAAAGGTTT  
5351  TGCGCCATTC GATGGTGTCC GGGATCTCGA CGCTCTCCCT TATGCGACTC  
5401  CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG  
5451  CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC  
5501  ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA  
5551  GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG  
5601  CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG  
5651  AGGATCGAGA TCTCGATCCC GCGAAAT