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pET-11c

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At a Glance

pET-11c, Image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
lac operator
ribosome binding site
Nde I cloning site
BamH I cloning site
lacI repressor ORF

Accession Number




Notes

In the pET system the sequence of interest is transformed into E. coli after being transcribed downstream from the T7 promoter and the gene 10 ribosome binding site. One can use IPTG induction of a chromosomally integrated cassette or infection with a polymerase-expressing bacteriophage lamba CE6 to achieve protien expression. After induction to E. coli the very active polymerase cause a high nimber of transcipts. Due to this phenomenon and high efficiency of translation, the target protein may become the majority of the cellular protein in only a matter of hours. The only differenvce between pET-11a, pET-11b and the plasmid pET-11c is that the ORF ends with an arginine instead of a different protein. [1]

History

The pET-11 plasmids are the improved form of the pET-3 plasmids. The lac operator has been placed in between the T7 promoter and the translation initiation sequences to produce a higher level of regulation and control. IPTG has to be present or the plasmid results in repression. These plasmids also include the lacIq gene to provide adequate levels of LacI repressor protiens. This shuts off the T7 polymerase gene expression along with the T7 promoter transcription.[2]

Sources

Agilent Genomics

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

  1. Bonin, C. "PNAS,Vol 94". 1996. Page 2085-9090.
  2. Bonin, C. "PNAS,Vol 94". 1996. Page 2085-9090

Sequence

THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGCTAGCATG  
 101  ACTGGTGGAC AGCAAATGGG TCGGATCCGG CTGCTAACAA AGCCCGAAAG  
 151  GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT  
 201  TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA  
 251  TATCCGGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA  
 301  TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG  
 351  TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA  
 401  ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT  
 451  TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT  
 501  GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC  
 551  GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG  
 601  CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA  
 651  GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG  
 701  CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA  
 751  GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT  
 801  CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA  
 851  TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT  
 901  GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA  
 951  CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA  
1001  CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG  
1051  GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT  
1101  TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG  
1151  AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA  
1201  GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT  
1251  AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG  
1301  GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA  
1351  TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC  
1401  AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG  
1451  CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG  
1501  ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT  
1551  TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT  
1601  TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA  
1651  GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT  
1701  TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG  
1751  TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT  
1801  GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA  
1851  GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC  
1901  TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT  
1951  ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG  
2001  CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA  
2051  CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC  
2101  GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG  
2151  AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC  
2201  CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG  
2251  TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG  
2301  GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT  
2351  CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC  
2401  GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC  
2451  GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT  
2501  CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT  
2551  AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG  
2601  CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG  
2651  CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT  
2701  GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT  
2751  AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA  
2801  TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT  
2851  GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG  
2901  CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA  
2951  CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT  
3001  ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC  
3051  AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA  
3101  GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT  
3151  AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA  
3201  AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG  
3251  CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT  
3301  AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA  
3351  TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG  
3401  CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG  
3451  CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG  
3501  TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC  
3551  GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG  
3601  GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA  
3651  AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG  
3701  CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG  
3751  GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG  
3801  AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA  
3851  CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG  
3901  CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG  
3951  CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC  
4001  GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT  
4051  ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT  
4101  GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA  
4151  TCGGTCGAGA TCCCGGTGCC TAATGAGTGA GCTAACTTAC ATTAATTGCG  
4201  TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA  
4251  TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC  
4301  AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT  
4351  CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC  
4401  CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG GATATAACAT  
4451  GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATAT CCGCACCAAC  
4501  GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT  
4551  CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC  
4601  ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC  
4651  TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC  
4701  GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG CGCGATTTGC  
4751  TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG TACCGTCTTC  
4801  ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG ACATCAAGAA  
4851  ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG  
4901  TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG  
4951  ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG  
5001  ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT AATCGCCGCG  
5051  ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG CAACGCCAAT  
5101  CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG TTGGGAATGT  
5151  AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT TTTCGCAGAA  
5201  ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT AAGAGACACC  
5251  GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCACA TTCACCACCC  
5301  TGAATTGACT CTCTTCCGGG CGCTATCATG CCATACCGCG AAAGGTTTTG  
5351  CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA TGCGACTCCT  
5401  GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC  
5451  GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC  
5501  GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT  
5551  GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA  
5601  ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG  
5651  GATCGAGATC TCGATCCCGC GAAAT