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pET-14b

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At a Glance

pET-14b, Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
His tag
MCS
T7 Terminator
Thrombin cleavage site

Accession Number




Notes

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
     241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
     301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
     361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
     421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
     481 cagcttcctt tcgggctttg ttagcagccg gatcctcgag catatggctg ccgcgcggca 
     541 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta 
     601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat 
     661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 
     721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 
     781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 
     841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 
     901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 
     961 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 
    1021 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 
    1081 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 
    1141 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 
    1201 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 
    1261 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 
    1321 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 
    1381 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 
    1441 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 
    1501 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 
    1561 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 
    1621 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 
    1681 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca 
    1741 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 
    1801 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 
    1861 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 
    1921 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 
    1981 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 
    2041 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 
    2101 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 
    2161 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 
    2221 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 
    2281 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 
    2341 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 
    2401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 
    2461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 
    2521 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 
    2581 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg 
    2641 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
    2701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
    2761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
    2821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
    2881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
    2941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
    3001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
    3061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
    3121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
    3181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
    3241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
    3301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
    3361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
    3421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
    3481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
    3541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
    3601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
    3661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
    3721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
    3781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
    3841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
    3901 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
    3961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
    4021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
    4081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
    4141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
    4201 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
    4261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
    4321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
    4381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
    4441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
    4501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
    4561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
    4621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a