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pET-15b

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At a Glance

pET-15b Image provided by Novagen

Type

Cloning/Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter [1]
T7 transcription start
His-Tag [1][2][3][4][5]
MCS
T7 Terminator
lacI

Accession Number




Notes

The pET-15b[1][2][3][4][5][6] vector possesses an His•Tag at the N-terminus, a subsequent [thrombin cleavage site][2] and three cloning sites.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 Björk, P et al. (2006) Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites. Mol. Biol. Cell 17 32-42 PubMed EcoliWiki page
  2. 2.0 2.1 2.2 Deikus, G & Bechhofer, DH (2007) Initiation of decay of Bacillus subtilis trp leader RNA. J. Biol. Chem. 282 20238-44 PubMed EcoliWiki page
  3. 3.0 3.1 Unno, H et al. (2007) Structural and mutational studies of anthocyanin malonyltransferases establish the features of BAHD enzyme catalysis. J. Biol. Chem. 282 15812-22 PubMed EcoliWiki page
  4. 4.0 4.1 Praetorius-Ibba, M et al. (2007) Functional association between three archaeal aminoacyl-tRNA synthetases. J. Biol. Chem. 282 3680-7 PubMed EcoliWiki page
  5. 5.0 5.1 Yatsenko, AS et al. (2007) A putative Src homology 3 domain binding motif but not the C-terminal dystrophin WW domain binding motif is required for dystroglycan function in cellular polarity in Drosophila. J. Biol. Chem. 282 15159-69 PubMed EcoliWiki page
  6. Kaustov, L et al. (2007) The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain of p53. J. Biol. Chem. 282 11300-7 PubMed EcoliWiki page

[1] [2] [3] [4] [5] [6]

Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 
     241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 
     301 cgggctttgt tagcagccgg atcctcgagc atatggctgc cgcgcggcac caggccgctg 
     361 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa 
     421 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa 
     481 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc 
     541 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct 
     601 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc 
     661 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac 
     721 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga 
     781 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga 
     841 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta 
     901 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc   
     961 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt 
    1021 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc 
    1081 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc 
    1141 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca 
    1201 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga 
    1261 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga 
    1321 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga 
    1381 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt 
    1441 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc 
    1501 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat 
    1561 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg 
    1621 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg 
    1681 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga 
    1741 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc 
    1801 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc 
    1861 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 
    1921 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc 
    1981 attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc agctccttcc 
    2041 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 
    2101 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga 
    2161 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag 
    2221 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca 
    2281 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct 
    2341 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc 
    2401 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta 
    2461 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga 
    2521 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg 
    2581 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct 
    2641 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa 
    2701 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac 
    2761 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc 
    2821 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat 
    2881 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg 
    2941 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac 
    3001 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 
    3061 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca 
    3121 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac 
    3181 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc 
    3241 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt 
    3301 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca 
    3361 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc 
    3421 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
    3481 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
    3541 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 
    3601 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac 
    3661 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 
    3721 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
    3781 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
    3841 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
    3901 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
    3961 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
    4021 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
    4081 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
    4141 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
    4201 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
    4261 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
    4321 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
    4381 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
    4441 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
    4501 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
    4561 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
    4621 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
    4681 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
    4741 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
    4801 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
    4861 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
    4921 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 
    4981 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
    5041 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
    5101 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
    5161 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
    5221 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 
    5281 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
    5341 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
    5401 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
    5461 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
    5521 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
    5581 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 
    5641 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 
    5701 ttcaagaa


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