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pET-16b

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At a Glance

pET-16b, Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
T7 transc start
T7 terminator
His•Tag
MCS
lacI

Accession Number




Notes

The pET-16b vector includes an N-terminal His•Tag sequence, a Factor Xa site and three cloning sites. T7 expression region is reversed on circular map.

History

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Sources

Novagen

References

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Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 
     241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 
     301 cgggctttgt tagcagccgg atcctcgagc atatgacgac cttcgatatg gccgctgctg 
     361 tgatgatgat gatgatgatg atgatgatgg cccatggtat atctccttct taaagttaaa 
     421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat 
     481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 
     541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 
     601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 
     661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 
     721 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 
     781 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc 
     841 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga 
     901 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc 
     961 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg 
    1021 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct 
    1081 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg 
    1141 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt 
    1201 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca 
    1261 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc 
    1321 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt 
    1381 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc 
    1441 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca 
    1501 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca 
    1561 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct 
    1621 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt 
    1681 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca 
    1741 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca 
    1801 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc 
    1861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 
    1921 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca 
    1981 ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 
    2041 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 
    2101 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 
    2161 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 
    2221 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 
    2281 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg 
    2341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca 
    2401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc 
    2461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg 
    2521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc 
    2581 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca 
    2641 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt 
    2701 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg 
    2761 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct 
    2821 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 
    2881 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 
    2941 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 
    3001 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 
    3061 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 
    3121 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 
    3181 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 
    3241 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 
    3301 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 
    3361 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 
    3421 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 
    3481 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 
    3541 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 
    3601 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 
    3661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 
    3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
    3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
    3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
    3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
    3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
    4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
    4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
    4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
    4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
    4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
    4321 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
    4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
    4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
    4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
    4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
    4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
    4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
    4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
    4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
    4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
    4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 
    4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
    5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
    5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
    5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
    5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 
    5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
    5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
    5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
    5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
    5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
    5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 
    5641 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 
    5701 gtcttcaaga a