PortEco logo

pET-19b

From EcoliWiki
Jump to: navigation, search

At a Glance

pET-19b, Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
T7 transcription start
T7 terminator
His•Tag coding sequence
MCS
lacI coding sequence

Accession Number




Notes

The pET-19b vector includes an N-terminal His•Tag sequence, an enterokinase cleavage site and three cloning sites.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

[1] [2]

Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 
     241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 
     301 cgggctttgt tagcagccgg atcctcgagc atatgcttgt cgtcgtcgtc gatatggccg 
     361 ctgctgtgat gatgatgatg atgatgatga tgatggccca tggtatatct ccttcttaaa 
     421 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag 
     481 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc 
     541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 
     601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 
     661 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 
     721 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 
     781 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 
     841 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 
     901 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 
     961 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 
    1021 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 
    1081 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 
    1141 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 
    1201 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 
    1261 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 
    1321 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 
    1381 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 
    1441 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 
    1501 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 
    1561 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 
    1621 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 
    1681 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 
    1741 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 
    1801 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 
    1861 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
    1921 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 
    1981 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 
    2041 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 
    2101 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 
    2161 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 
    2221 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 
    2281 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct 
    2341 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc 
    2401 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg 
    2461 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg 
    2521 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct 
    2581 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg 
    2641 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag 
    2701 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag 
    2761 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt 
    2821 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga 
    2881 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc 
    2941 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc 
    3001 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac 
    3061 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg 
    3121 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc 
    3181 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa 
    3241 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat 
    3301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc 
    3361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag 
    3421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
    3481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
    3541 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 
    3601 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt 
    3661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 
    3721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
    3781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
    3841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
    3901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
    3961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
    4021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
    4081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
    4141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
    4201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
    4261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
    4321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
    4381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
    4441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
    4501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
    4561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
    4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
    4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
    4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
    4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
    4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
    4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 
    4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
    5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
    5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
    5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
    5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 
    5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
    5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
    5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
    5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
    5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
    5581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
    5641 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 
    5701 cctttcgtct tcaagaa


Cite error: <ref> tags exist, but no <references/> tag was found