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pET-20b(+)

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At a Glance

pET-20b(+), Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

f1

pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter[1]
T7 transcription start
T7 terminator
pelB coding sequence
MCS
His•Tag coding sequence

Accession Number




Notes

The pET-20b(+) vector includes an N-terminal pelB signal sequence for potential periplasmic localization and an optional C-terminal His•Tag sequence.

History

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Sources

Novagen

References

[1]

  1. 1.0 1.1 Gunasekera, D & Kemp, RG (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expr. Purif. 16 448-53 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 
     241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 
     301 cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc cctatagtga 
     361 gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg 
     421 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta 
     481 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc 
     541 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 
     601 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 
     661 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 
     721 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 
     781 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 
     841 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 
     901 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 
     961 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 
    1021 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
    1081 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
    1141 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 
    1201 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 
    1261 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
    1321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
    1381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
    1441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
    1501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
    1561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
    1621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
    1681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
    1741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
    1801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
    1861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
    1921 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
    1981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
    2041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
    2101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
    2161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
    2221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
    2281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
    2341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
    2401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
    2461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
    2521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca 
    2581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
    2641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
    2701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
    2761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
    2821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
    2881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
    2941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
    3001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
    3061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
    3121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
    3181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
    3241 tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 
    3301 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 
    3361 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 
    3421 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 
    3481 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 
    3541 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 
    3601 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 
    3661 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca