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pET-21b(+)

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At a Glance

pET-21b(+), Image made with PlasMapper

Type

Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
T7 transcription start
T7 terminator
T7•Tag coding sequence
MCS
His•Tag coding sequence
lacI coding sequence

Accession Number




Notes

The map for pET-21b(+) is the same as pET-21a(+) except that pET-21b(+) is a 5442bp plasmid; 1bp less from each site beyond BamH1 at 198.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1]

  1. McMurry, LM & Levy, SB (1995) The NH2-terminal half of the Tn10-specified tetracycline efflux protein TetA contains a dimerization domain. J. Biol. Chem. 270 22752-7 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 
     241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 
     301 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 
     361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 
     421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 
     481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 
     541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 
     601 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 
     661 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 
     721 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 
     781 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 
     841 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 
     901 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 
     961 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 
    1021 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 
    1081 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 
    1141 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 
    1201 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 
    1261 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 
    1321 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 
    1381 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 
    1441 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 
    1501 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 
    1561 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 
    1621 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 
    1681 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
    1741 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
    1801 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 
    1861 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 
    1921 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 
    1981 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 
    2041 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 
    2101 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc 
    2161 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac 
    2221 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa 
    2281 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct 
    2341 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta 
    2401 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca 
    2461 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag 
    2521 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa 
    2581 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc 
    2641 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg 
    2701 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc 
    2761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
    2821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
    2881 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 
    2941 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa 
    3001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 
    3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
    3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
    3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
    3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
    3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
    3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
    3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
    3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
    3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
    3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
    3661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
    3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
    3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
    3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
    3901 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
    3961 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
    4021 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
    4081 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
    4141 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
    4201 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
    4261 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 
    4321 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
    4381 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
    4441 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
    4501 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
    4561 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
    4621 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
    4681 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
    4741 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
    4801 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
    4861 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
    4921 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 
    4981 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 
    5041 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 
    5101 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 
    5161 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 
    5221 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 
    5281 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 
    5341 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 
    5401 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca