PortEco logo

pET-21d(+)

From EcoliWiki
Jump to: navigation, search

At a Glance

pET-21d(+), Image made with PlasMapper

Type

Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
T7 transcription start
T7 terminator
T7•Tag coding sequence
MCS
His•Tag coding sequence
lacI coding sequence


Accession Number




Notes

The map for pET-21d(+) is the same as pET-21a(+) except that pET-21d(+) is a 5440bp plasmid. The BamH I site is in the same reading frame as in pET-21c(+). An Nco I site is in place of the Nde I site with a total 1bp deletion at position 238 of pET-21c(+). As a result, Nco I cuts pET21d(+) at 234, and Nhe I cuts at 229. For the remaining sites, subtract 3bp from each site beyond position 239 in pET-21a(+). Nde I does not cut pET-21d(+). Sty I is not unique in pET-21d(+).

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1] [2] [3]

  1. Kassavetis, GA et al. (2005) Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB. Proc. Natl. Acad. Sci. U.S.A. 102 15406-11 PubMed EcoliWiki page
  2. Liu, G et al. (2005) High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases. Protein Sci. 14 1597-608 PubMed EcoliWiki page
  3. Webb, BL et al. (1995) An interaction between the Escherichia coli RecF and RecR proteins dependent on ATP and double-stranded DNA. J. Biol. Chem. 270 31397-404 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt 
     241 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 
     301 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg 
     361 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 
     421 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 
     481 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 
     541 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 
     601 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc 
     661 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag 
     721 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 
     781 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 
     841 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 
     901 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 
     961 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 
    1021 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 
    1081 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 
    1141 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 
    1201 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 
    1261 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 
    1321 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 
    1381 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 
    1441 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 
    1501 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 
    1561 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 
    1621 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 
    1681 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 
    1741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 
    1801 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga 
    1861 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 
    1921 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt 
    1981 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc 
    2041 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc 
    2101 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg 
    2161 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg 
    2221 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca 
    2281 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc 
    2341 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca 
    2401 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc 
    2461 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta 
    2521 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat 
    2581 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc 
    2641 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa 
    2701 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc 
    2761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 
    2821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 
    2881 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 
    2941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat 
    3001 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 
    3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
    3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
    3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
    3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
    3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
    3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
    3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
    3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
    3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
    3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
    3661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
    3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
    3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
    3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
    3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
    3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
    4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
    4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
    4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
    4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
    4261 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 
    4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
    4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
    4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
    4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
    4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
    4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
    4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
    4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
    4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
    4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
    4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt 
    4981 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 
    5041 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 
    5101 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 
    5161 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 
    5221 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 
    5281 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 
    5341 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 
    5401 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca