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pET-22b(+)

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At a Glance

pET-22b(+), Image made with PlasMapper

Type

Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
pelB coding sequence
Multiple cloning sites (NcoI -XhoI)
His Tag coding sequence
lacI coding sequence

Accession Number




Notes

The pET-22b(+) vector has an N-terminal pelB signal sequence for potential periplasmic localization, as well as optional C-terminal His•Tag sequence. The sequence is numbered by the pBR322 convention, thus the T7 expression region is reversed on the vector map.

History

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Sources

Novagen

References

[1] [2] [3] [4] [5] [6] [7]

  1. Seybert, A et al. (2002) Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Nucleic Acids Res. 30 4329-38 PubMed EcoliWiki page
  2. Barillà, D et al. (2001) Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 98 445-50 PubMed EcoliWiki page
  3. Koo, JH et al. (2000) The malonate decarboxylase operon of Acinetobacter calcoaceticus KCCM 40902 is regulated by malonate and the transcriptional repressor MdcY. J. Bacteriol. 182 6382-90 PubMed EcoliWiki page
  4. Lin, Z et al. () Functional expression of horseradish peroxidase in E. coli by directed evolution. Biotechnol. Prog. 15 467-71 PubMed EcoliWiki page
  5. MacBeath, G et al. (1998) Redesigning enzyme topology by directed evolution. Science 279 1958-61 PubMed EcoliWiki page
  6. MacKenzie-Graham, AJ et al. (1997) Myelin protein expression is increased in lymph nodes of mice with relapsing experimental autoimmune encephalomyelitis. J. Immunol. 159 4602-10 PubMed EcoliWiki page
  7. Lambalot, RH & Walsh, CT (1995) Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase. J. Biol. Chem. 270 24658-61 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 
     241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 
     301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 
     361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc 
     421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 
     481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 
     541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 
     601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 
     661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat 
     721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt 
     781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 
     841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 
     901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 
     961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 
    1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 
    1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 
    1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 
    1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 
    1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 
    1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 
    1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 
    1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 
    1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 
    1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 
    1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 
    1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 
    1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
    1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
    1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca 
    1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg 
    1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg 
    2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct 
    2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc 
    2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga 
    2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg 
    2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg 
    2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta 
    2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat 
    2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg 
    2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta 
    2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt 
    2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca 
    2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag 
    2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt 
    2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
    2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
    2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 
    3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 
    3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
    3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
    3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
    3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
    3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
    3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
    3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
    3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
    3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
    3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
    3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
    3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
    3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
    3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
    3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
    3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
    4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
    4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
    4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
    4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
    4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
    4321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 
    4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
    4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
    4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
    4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
    4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
    4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
    4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
    4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
    4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
    4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
    4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt 
    5041 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
    5101 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
    5161 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
    5221 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
    5281 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
    5341 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
    5401 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
    5461 aatgcgccgc tacagggcgc gtcccattcg cca