PortEco logo

pET-23b(+)

From EcoliWiki
Jump to: navigation, search

At a Glance

pET-23b(+), Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
pelB coding sequence
Multiple cloning sites (NcoI-XhoI)
His Tag coding sequence
lacI coding sequence

Accession Number




Notes

The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. The map for pET-23b(+) is the same as pET-23a(+).

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1] [2] [3] [4] [5] [6] [7] [8] [9]

  1. Heurgué-Hamard, V et al. (2005) The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene. J. Biol. Chem. 280 2439-45 PubMed EcoliWiki page
  2. Karla, A et al. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. J. Biol. Chem. 280 6731-41 PubMed EcoliWiki page
  3. Li, L et al. (2005) Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of Aspen. Plant Cell Physiol. 46 1073-82 PubMed EcoliWiki page
  4. Mata-Greenwood, E et al. (2005) Cyclic stretch increases VEGF expression in pulmonary arterial smooth muscle cells via TGF-beta1 and reactive oxygen species: a requirement for NAD(P)H oxidase. Am. J. Physiol. Lung Cell Mol. Physiol. 289 L288-9 PubMed EcoliWiki page
  5. Szweda, P et al. (2005) New effective sources of the Staphylococcus simulans lysostaphin. J. Biotechnol. 117 203-13 PubMed EcoliWiki page
  6. Gu, D et al. (2004) Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. J. Biol. Chem. 279 31171-6 PubMed EcoliWiki page
  7. Orelle, C et al. (2003) The conserved glutamate residue adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-binding cassette transporter BmrA. J. Biol. Chem. 278 47002-8 PubMed EcoliWiki page
  8. Bassham, DC et al. (2000) AtVPS45 complex formation at the trans-Golgi network. Mol. Biol. Cell 11 2251-65 PubMed EcoliWiki page
  9. Dietrich, JB et al. (1991) Expression of mammalian tyrosine aminotransferase in Saccharomyces cerevisiae and Escherichia coli. Purification to homogeneity and characterization of the enzyme overproduced in the bacteria. Eur. J. Biochem. 201 399-407 PubMed EcoliWiki page

Sequence

        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
      181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 
      241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt tgtggtctcc 
      301 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg ggcagcgttg ggtcctggcc 
      361 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 
      421 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 
      481 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 
      541 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 
      601 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 
      661 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 
      721 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 
      781 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 
      841 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 
      901 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 
      961 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     1021 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     1081 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 
     1141 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 
     1201 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 
     1261 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     1321 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     1381 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     1441 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     1501 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     1561 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     1621 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     1681 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     1741 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     1801 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     1861 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     1921 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     1981 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     2041 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     2101 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     2161 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     2221 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     2281 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     2341 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     2401 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     2461 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     2521 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     2581 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     2641 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     2701 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     2761 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     2821 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     2881 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     2941 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     3001 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     3061 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     3121 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     3181 cccgaaaagt gccacctgaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt 
     3241 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 
     3301 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 
     3361 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 
     3421 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 
     3481 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 
     3541 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 
     3601 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt 
     3661 cgcca