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pET-24c(+)

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At a Glance

pET-24c(+), Image made with PlasMapper

Type

Expression

Origin of Replication

f1, pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 promoter
T7•Tag coding sequence
Multiple cloning sites
T7 terminator
lacI coding sequence

Accession Number




Notes

The pET-24a-d(+) vectors possess an N-terminal sequence that codes for a T7 Tag. It also comes with an optional C-terminal His Tag sequence. The only difference between each individual plasmid from a-d is the selectable marker. In pET-24b(+) the selectable marker is kanamycin. On the vector ap the T7 repression region is opposite a normal region meaning it is reversed. This is a characteristic of the BR322 numbering convention. When exposed to helper phage, the fl origin produces virions containing single-dtranded DNA. These single stranded DNA correspond to the coding strand. The virions are a product of the orientation of the f1 origin.

History

All pET-24a-d(+) plasmids have the same structure and vector map. They differ in their selectable markers.

Sources

Novagen

References

See Help:References for how to manage references in EcoliWiki.


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 
     241 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 
     301 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 
     361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 
     421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 
     481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 
     541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 
     601 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 
     661 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 
     721 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 
     781 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 
     841 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 
     901 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 
     961 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 
    1021 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 
    1081 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 
    1141 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 
    1201 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 
    1261 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 
    1321 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 
    1381 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 
    1441 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 
    1501 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 
    1561 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 
    1621 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 
    1681 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
    1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
    1801 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 
    1861 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 
    1921 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 
    1981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 
    2041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 
    2101 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 
    2161 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 
    2221 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 
    2281 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 
    2341 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 
    2401 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 
    2461 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 
    2521 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 
    2581 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 
    2641 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 
    2701 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 
    2761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 
    2821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 
    2881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 
    2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 
    3001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 
    3061 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
    3121 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
    3181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
    3241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
    3301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
    3361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
    3421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
    3481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
    3541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
    3601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
    3661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
    3721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
    3781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
    3841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg 
    3901 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 
    3961 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 
    4021 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 
    4081 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 
    4141 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 
    4201 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 
    4261 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 
    4321 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 
    4381 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 
    4441 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 
    4501 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 
    4561 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 
    4621 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 
    4681 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 
    4741 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca 
    4801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat 
    4861 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 
    4921 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 
    4981 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 
    5041 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 
    5101 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 
    5161 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 
    5221 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 
    5281 gccgctacag ggcgcgtccc attcgcca