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pET-26b(+)

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At a Glance

pET-26b(+), Image provided by Novagen

Type

Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

C terminal His Tag
T7 Promoter
MCS
lacI
pelB

Accession Number




Notes

The pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization in addition to an optional C-terminal His•Tag sequence. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12]

  1. Funa, N et al. (2007) Pentaketide resorcylic acid synthesis by type III polyketide synthase from Neurospora crassa. J. Biol. Chem. 282 14476-81 PubMed EcoliWiki page
  2. Li, Y et al. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Appl. Environ. Microbiol. 73 3165-72 PubMed EcoliWiki page
  3. Heraud, JM et al. (2006) Subunit recombinant vaccine protects against monkeypox. J. Immunol. 177 2552-64 PubMed EcoliWiki page
  4. Brouns, SJ et al. (2005) Engineering a selectable marker for hyperthermophiles. J. Biol. Chem. 280 11422-31 PubMed EcoliWiki page
  5. Funa, N et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. J. Biol. Chem. 280 14514-23 PubMed EcoliWiki page
  6. Ishii, R et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. J. Biol. Chem. 280 14138-44 PubMed EcoliWiki page
  7. Li, H et al. (2005) Crystal structures of T cell receptor (beta) chains related to rheumatoid arthritis. Protein Sci. 14 3025-38 PubMed EcoliWiki page
  8. Tsubouchi, T et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. J. Biol. Chem. 280 18511-6 PubMed EcoliWiki page
  9. Vincent, F et al. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? J. Biol. Chem. 280 19649-55 PubMed EcoliWiki page
  10. Chen, YH et al. (2004) Structural basis of the alpha1-beta subunit interaction of voltage-gated Ca2+ channels. Nature 429 675-80 PubMed EcoliWiki page
  11. Teartasin, W et al. (2004) Expression, purification and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallogr. D Biol. Crystallogr. 60 1137-8 PubMed EcoliWiki page
  12. Goldberg, SD et al. (2003) Identification of residues critical for catalysis in a class C beta-lactamase by combinatorial scanning mutagenesis. Protein Sci. 12 1633-45 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 
     241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 
     301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 
     361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc 
     421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 
     481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 
     541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 
     601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 
     661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat 
     721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt 
     781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 
     841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 
     901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 
     961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 
    1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 
    1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 
    1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 
    1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 
    1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 
    1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 
    1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 
    1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 
    1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 
    1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 
    1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 
    1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 
    1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
    1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
    1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca 
    1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg 
    1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg 
    2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct 
    2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc 
    2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga 
    2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg 
    2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg 
    2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta 
    2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat 
    2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg 
    2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta 
    2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt 
    2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca 
    2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag 
    2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt 
    2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 
    2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 
    2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 
    3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 
    3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
    3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
    3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
    3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
    3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
    3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
    3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
    3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
    3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
    3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
    3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
    3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
    3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
    3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
    3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta 
    3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag 
    4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 
    4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt 
    4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 
    4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 
    4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga 
    4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 
    4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 
    4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 
    4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 
    4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 
    4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 
    4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 
    4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 
    4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
    4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa 
    4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
    4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
    5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
    5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
    5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
    5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
    5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
    5341 agggcgcgtc ccattcgcca