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pET-28c(+)

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At a Glance

pET-28a-c(+), image provided by Novagen

Type

Cloning, Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
His Tag
lacI
MCS

Accession Number




Notes

The pET-28c(+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, the T7 terminator primer should be used when performing single stranded sequencing.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]

  1. Crosson, S et al. (2005) Conserved modular design of an oxygen sensory/signaling network with species-specific output. Proc. Natl. Acad. Sci. U.S.A. 102 8018-23 PubMed EcoliWiki page
  2. Futter, M et al. (2005) Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5). Proc. Natl. Acad. Sci. U.S.A. 102 3489-94 PubMed EcoliWiki page
  3. Georgescu, SP et al. (2005) Modulator recognition factor 1, an AT-rich interaction domain family member, is a novel corepressor for estrogen receptor alpha. Mol. Endocrinol. 19 2491-501 PubMed EcoliWiki page
  4. Mori, T et al. (2005) Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp. J. Biol. Chem. 280 9345-53 PubMed EcoliWiki page
  5. Kurebayashi, S et al. (2004) Selective LXXLL peptides antagonize transcriptional activation by the retinoid-related orphan receptor RORgamma. Biochem. Biophys. Res. Commun. 315 919-27 PubMed EcoliWiki page
  6. Zhong, H et al. (2002) The heteromeric cyclic nucleotide-gated channel adopts a 3A:1B stoichiometry. Nature 420 193-8 PubMed EcoliWiki page
  7. Peñas, MM et al. (1998) Identification, characterization, and In situ detection of a fruit-body-specific hydrophobin of Pleurotus ostreatus. Appl. Environ. Microbiol. 64 4028-34 PubMed EcoliWiki page
  8. Qian, F et al. (1997) PKD1 interacts with PKD2 through a probable coiled-coil domain. Nat. Genet. 16 179-83 PubMed EcoliWiki page
  9. Zhou, H et al. (1997) CIITA-dependent and -independent class II MHC expression revealed by a dominant negative mutant. J. Immunol. 158 4741-9 PubMed EcoliWiki page
  10. Hawdon, JM et al. (1996) Cloning and characterization of Ancylostoma-secreted protein. A novel protein associated with the transition to parasitism by infective hookworm larvae. J. Biol. Chem. 271 6672-8 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 
     241 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt 
     301 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 
     361 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg 
     421 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 
     481 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 
     541 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 
     601 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 
     661 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc 
     721 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag 
     781 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 
     841 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 
     901 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 
     961 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 
    1021 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 
    1081 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 
    1141 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 
    1201 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 
    1261 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 
    1321 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 
    1381 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 
    1441 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 
    1501 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 
    1561 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 
    1621 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 
    1681 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 
    1741 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 
    1801 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 
    1861 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga 
    1921 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 
    1981 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt 
    2041 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc 
    2101 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc 
    2161 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg 
    2221 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg 
    2281 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca 
    2341 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc 
    2401 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca 
    2461 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc 
    2521 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta 
    2581 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat 
    2641 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc 
    2701 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa 
    2761 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc 
    2821 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 
    2881 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 
    2941 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 
    3001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat 
    3061 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 
    3121 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
    3181 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
    3241 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
    3301 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
    3361 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
    3421 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
    3481 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
    3541 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
    3601 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
    3661 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
    3721 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
    3781 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
    3841 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
    3901 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca ataaaactgt 
    3961 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt 
    4021 gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 
    4081 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc 
    4141 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 
    4201 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 
    4261 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat 
    4321 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 
    4381 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg 
    4441 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 
    4501 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac 
    4561 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 
    4621 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 
    4681 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 
    4741 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 
    4801 ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
    4861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt 
    4921 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 
    4981 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 
    5041 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 
    5101 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 
    5161 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 
    5221 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 
    5281 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 
    5341 ccgctacagg gcgcgtccca ttcgcca