PortEco logo

pET-29c(+)

From EcoliWiki
Jump to: navigation, search

At a Glance

pET-29c(+), Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

f1, pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 promoter
S tag
MCS
His tag
lacI

Accession Number




Notes

The pET-29a-c(+) vectors carry an N-terminal S•Tag™/thrombin configuration plus an optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

See Help:References for how to manage references in EcoliWiki.


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccacagata tcccatggaa ccgcgtggca ccagggtacc 
     241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatatg 
     301 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 
     361 aattccccta tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac 
     421 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 
     481 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 
     541 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat 
     601 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 
     661 atgcaggagt cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac 
     721 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa 
     781 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 
     841 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 
     901 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 
     961 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 
    1021 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 
    1081 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 
    1141 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 
    1201 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 
    1261 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 
    1321 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 
    1381 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 
    1441 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 
    1501 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 
    1561 gcgcgttggt gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta 
    1621 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 
    1681 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 
    1741 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 
    1801 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 
    1861 gcaacgcaat taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct 
    1921 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg 
    1981 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg 
    2041 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg 
    2101 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt 
    2161 tcggcgagaa gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc 
    2221 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat 
    2281 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa 
    2341 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc 
    2401 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac 
    2461 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc 
    2521 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat 
    2581 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca 
    2641 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg 
    2701 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg 
    2761 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct 
    2821 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 
    2881 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 
    2941 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 
    3001 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt 
    3061 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 
    3121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
    3181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
    3241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
    3301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
    3361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
    3421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
    3481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
    3541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
    3601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
    3661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
    3721 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
    3781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
    3841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
    3901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa 
    3961 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 
    4021 gtcttgctct aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg 
    4081 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga 
    4141 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga 
    4201 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat 
    4261 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca 
    4321 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct 
    4381 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg 
    4441 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga 
    4501 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt 
    4561 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga 
    4621 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga 
    4681 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt 
    4741 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga 
    4801 tgagtttttc taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata 
    4861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta 
    4921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 
    4981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 
    5041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 
    5101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 
    5161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 
    5221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 
    5281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 
    5341 atgcgccgct acagggcgcg tcccattcgc ca