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pET-30a(+)

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At a Glance

pET-30a(+) Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

f1, pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
lac operator
N-terminal
His•Tag/thrombin/S•Tag/enterokinase configuration
MCS
optional C-terminal His•Tag
lacI coding sequence

Accession Number




Notes

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc gtcggtaccc 
     241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca 
     301 gaaccgcgtg gcaccagacc agaagaatga tgatgatgat ggtgcatatg tatatctcct 
     361 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta 
     421 tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca 
     481 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 
     541 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 
     601 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 
     661 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 
     721 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt 
     781 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg 
     841 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 
     901 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 
     961 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 
    1021 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 
    1081 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 
    1141 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 
    1201 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 
    1261 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 
    1321 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 
    1381 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 
    1441 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 
    1501 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 
    1561 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 
    1621 gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 
    1681 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 
    1741 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 
    1801 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
    1861 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
    1921 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt 
    1981 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 
    2041 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg 
    2101 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat 
    2161 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa 
    2221 gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc tcctgtcgtt 
    2281 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg 
    2341 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat 
    2401 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat 
    2461 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt 
    2521 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac 
    2581 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg 
    2641 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc 
    2701 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat 
    2761 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc 
    2821 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg 
    2881 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
    2941 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
    3001 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 
    3061 tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc 
    3121 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 
    3181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
    3241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
    3301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
    3361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
    3421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
    3481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
    3541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
    3601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
    3661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
    3721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 
    3781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
    3841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
    3901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
    3961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa actgtctgct 
    4021 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctct 
    4081 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 
    4141 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag 
    4201 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 
    4261 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 
    4321 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa 
    4381 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 
    4441 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 
    4501 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 
    4561 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 
    4621 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 
    4681 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 
    4741 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 
    4801 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 
    4861 taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
    4921 ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt 
    4981 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 
    5041 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 
    5101 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 
    5161 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 
    5221 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 
    5281 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 
    5341 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 
    5401 acagggcgcg tcccattcgc ca