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pET-30b(+)

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At a Glance

pET-30b(+) Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
N-terminal His•Tag®/thrombin/S•Tag™/enterokinase configuration
Optional C-terminal His•Tag sequence
MCS
lacI coding sequence

Accession Number




Notes

The map for pET-30b(+) is the same for pET-30a(+) except that it has has 5421bp. Subtract 1bp from each site beyond Bam HI at 198.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgatatcg ccatggcctt gtcgtcgtcg tcggtaccca 
     241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccag 
     301 aaccgcgtgg caccagacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt 
     361 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat 
     421 agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat 
     481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac 
     541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat 
     601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct 
     661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc 
     721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta 
     781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt 
     841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 
     901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 
     961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 
    1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 
    1081 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 
    1141 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 
    1201 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 
    1261 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg 
    1321 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag 
    1381 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca 
    1441 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt 
    1501 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca 
    1561 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg 
    1621 cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt 
    1681 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc 
    1741 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa 
    1801 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 
    1861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 
    1921 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc 
    1981 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 
    2041 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc 
    2101 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc 
    2161 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag 
    2221 caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg 
    2281 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc 
    2341 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg 
    2401 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt 
    2461 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta 
    2521 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc 
    2581 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt 
    2641 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct 
    2701 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc 
    2761 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca 
    2821 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt 
    2881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 
    2941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 
    3001 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 
    3061 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca 
    3121 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 
    3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
    3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
    3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
    3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
    3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
    3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
    3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
    3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
    3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
    3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
    3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
    3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
    3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
    3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt 
    4021 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta 
    4081 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 
    4141 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 
    4201 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 
    4261 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 
    4321 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag 
    4381 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 
    4441 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 
    4501 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 
    4561 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt 
    4621 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 
    4681 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 
    4741 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 
    4801 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 
    4861 aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
    4921 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta 
    4981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 
    5041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 
    5101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 
    5161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 
    5221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 
    5281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 
    5341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 
    5401 cagggcgcgt cccattcgcc a