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pET-30c(+)

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At a Glance

pET-30c(+) Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
N-terminal His•Tag/thrombin/S•Tag/enterokinase configuration
Optional C-terminal His•Tag sequence
MCS
lacI coding sequence

Accession Number




Notes

The map for pET-30c(+) is the same as pET-30a(+), except that pET-30c(+) is a 5432 bp plasmid; add 1bp to each site beyond BamHI at 198.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc 
     241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc 
     301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat gtatatctcc 
     361 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct 
     421 atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta cgccggacgc 
     481 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc 
     541 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt 
     601 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc 
     661 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag 
     721 tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg 
     781 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac 
     841 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 
     901 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 
     961 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 
    1021 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 
    1081 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 
    1141 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 
    1201 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 
    1261 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 
    1321 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 
    1381 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 
    1441 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 
    1501 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 
    1561 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 
    1621 tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 
    1681 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 
    1741 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 
    1801 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 
    1861 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 
    1921 ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct 
    1981 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 
    2041 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 
    2101 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 
    2161 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 
    2221 agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt 
    2281 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac 
    2341 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa 
    2401 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca 
    2461 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg 
    2521 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata 
    2581 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc 
    2641 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc 
    2701 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta 
    2761 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg 
    2821 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc 
    2881 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 
    2941 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 
    3001 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 
    3061 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg 
    3121 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 
    3181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
    3241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
    3301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
    3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
    3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
    3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 
    3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
    3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
    3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
    3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
    3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
    3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
    3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
    3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc 
    4021 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 
    4081 taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 
    4141 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 
    4201 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 
    4261 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 
    4321 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 
    4381 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 
    4441 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 
    4501 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 
    4561 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 
    4621 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 
    4681 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 
    4741 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 
    4801 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 
    4861 ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
    4921 gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt 
    4981 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 
    5041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 
    5101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 
    5161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 
    5221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 
    5281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 
    5341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 
    5401 tacagggcgc gtcccattcg cca