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pET-31b(+) DNA

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At a Glance

pET-31b(+), Image made with PlasMapper

Type

Cloning, Expression [1]

Origin of Replication

pBR322
f1

Copy #
Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
KSI coding sequence
His Tag
lacI coding sequence

Accession Number




Notes

pET-31b(+) is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein [1].

Unique AlwN I cloning site allows unidirectional insertion of tandemly repeated peptide coding regions separated by methionine codons.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

  1. 1.0 1.1 Kuliopulos, A et al. (1994) Localization of the affinity peptide-substrate inactivator site on recombinant vitamin K-dependent carboxylase. J. Biol. Chem. 269 21364-70 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagcagcatc tggcatgcgt 
     181 gaatattctt ctcgccaaac aaggcgcgga tgctcaccac cttgccggcg ccattgaagc 
     241 gaaagtgatc gatgggcgca actacggtct tgcggccctg atactcgaag ctgacggtga 
     301 aagcgaaggc cgcttcgttg gcgaccgcgc gtacctcctg cgtcagctcc accgccaaag 
     361 gcagtttgag cgagttggcg taaaactcac gaatcgcagc cgtaccggac ctgggctcgg 
     421 aacccacggg gtcttccacc gtggcgtcat cggcaaacag cgcgacgatg ccgtccagat 
     481 cgccggcatt gagcgcagcc acaaagcgct gtaccacggc ggtgatgtgt tctggggtat 
     541 gcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc 
     601 cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc 
     661 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat 
     721 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt 
     781 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg 
     841 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc 
     901 ctaatgcagg agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa 
     961 aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt 
    1021 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc 
    1081 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc 
    1141 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc 
    1201 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc 
    1261 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg 
    1321 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg 
    1381 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac 
    1441 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt 
    1501 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca 
    1561 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg 
    1621 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag 
    1681 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc 
    1741 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg 
    1801 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg 
    1861 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt 
    1921 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt 
    1981 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc 
    2041 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 
    2101 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc 
    2161 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg 
    2221 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct 
    2281 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg 
    2341 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac 
    2401 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg cgcatgatcg 
    2461 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 
    2521 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 
    2581 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 
    2641 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 
    2701 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 
    2761 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 
    2821 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 
    2881 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca 
    2941 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 
    3001 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 
    3061 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 
    3121 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 
    3181 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 
    3241 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg 
    3301 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 
    3361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
    3421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
    3481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
    3541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
    3601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
    3661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
    3721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
    3781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
    3841 gagtccaacc cggtaagaca cgacttatcg ccactggcag ctggtaacag gattagcaga 
    3901 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
    3961 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
    4021 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
    4081 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
    4141 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
    4201 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
    4261 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
    4321 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
    4381 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
    4441 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
    4501 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
    4561 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 
    4621 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
    4681 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
    4741 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
    4801 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
    4861 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
    4921 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
    4981 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
    5041 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
    5101 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
    5161 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
    5221 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt 
    5281 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 
    5341 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 
    5401 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 
    5461 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 
    5521 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 
    5581 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 
    5641 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 
    5701 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca