PortEco logo

pET-32a(+) DNA

From EcoliWiki
Jump to: navigation, search

At a Glance

pBAD/His B, Image made with PlasMapper

Type

Cloning, Expression[1]

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
Trx Tag
His Tag
S Tag
MCS
lacI coding sequence

Accession Number




Notes

pET-32a(+) is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein [1].

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

  1. 1.0 1.1 LaVallie, ER et al. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology (N.Y.) 11 187-93 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgatatca gccatggcct tgtcgtcgtc gtcggtaccc 
     241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatacca 
     301 gaaccgcgtg gcaccagacc agaagaatga tgatgatgat ggtgcatatg gccagaacca 
     361 gaaccggcca ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcacccact 
     421 ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca 
     481 tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc 
     541 tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac 
     601 tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg 
     661 tcgtcagtca ggtgaataat tttatcgctc atatgtatat ctccttctta aagttaaaca 
     721 aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta 
     781 atttcgcggg atcgagatcg atctcgatcc tctacgccgg acgcatcgtg gccggcatca 
     841 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 
     901 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 
     961 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 
    1021 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 
    1081 gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc 
    1141 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca 
    1201 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 
    1261 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 
    1321 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 
    1381 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 
    1441 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 
    1501 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 
    1561 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 
    1621 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 
    1681 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 
    1741 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 
    1801 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 
    1861 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 
    1921 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga catctcggta 
    1981 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa 
    2041 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 
    2101 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 
    2161 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
    2221 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct 
    2281 cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc 
    2341 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 
    2401 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 
    2461 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 
    2521 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 
    2581 cggcatggcg gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg 
    2641 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag 
    2701 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg 
    2761 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg 
    2821 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg 
    2881 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc 
    2941 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct 
    3001 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc 
    3061 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt 
    3121 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc 
    3181 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg 
    3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 
    3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 
    3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 
    3421 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga 
    3481 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 
    3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 
    3601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
    3661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
    3721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
    3781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
    3841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
    3901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
    3961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
    4021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
    4081 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
    4141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
    4201 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
    4261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
    4321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
    4381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 
    4441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 
    4501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 
    4561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 
    4621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 
    4681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 
    4741 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 
    4801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 
    4861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 
    4921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 
    4981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 
    5041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 
    5101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 
    5161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 
    5221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 
    5281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 
    5341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 
    5401 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa 
    5461 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 
    5521 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 
    5581 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 
    5641 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 
    5701 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 
    5761 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 
    5821 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 
    5881 agggcgcgtc ccattcgcca