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pET-32b(+) DNA

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At a Glance

pET-32b(+), Image made with PlasMapper

Type

Cloning, Expression[1]

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
Trx Tag
His Tag
S Tag
MCS
lacI coding sequence

Accession Number




Notes

pET-32b(+) is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein[1]. The map for pET-32b(+) is the same as pET-32a(+) except that pET-32b(+) is a 5899bp plasmid; subtract 1bp from each site beyond BamHI at 198.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

  1. 1.0 1.1 LaVallie, ER et al. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology (N.Y.) 11 187-93 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccgatatcg ccatggcctt gtcgtcgtcg tcggtaccca 
     241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccag 
     301 aaccgcgtgg caccagacca gaagaatgat gatgatgatg gtgcatatgg ccagaaccag 
     361 aaccggccag gttagcgtcg aggaactctt tcaactgacc tttagacagt gcacccactt 
     421 tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat 
     481 atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct 
     541 gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact 
     601 ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt 
     661 cgtcagtcag gtgaataatt ttatcgctca tatgtatatc tccttcttaa agttaaacaa 
     721 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa 
     781 tttcgcggga tcgagatcga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac 
     841 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg 
     901 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 
     961 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 
    1021 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg 
    1081 tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc 
    1141 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag 
    1201 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 
    1261 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 
    1321 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 
    1381 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 
    1441 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 
    1501 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 
    1561 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 
    1621 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 
    1681 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 
    1741 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 
    1801 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 
    1861 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 
    1921 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggac atctcggtag 
    1981 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 
    2041 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 
    2101 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 
    2161 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
    2221 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc 
    2281 actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc 
    2341 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg 
    2401 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc 
    2461 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct 
    2521 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc 
    2581 ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc 
    2641 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc 
    2701 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt 
    2761 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc 
    2821 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg 
    2881 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc 
    2941 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc 
    3001 tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca 
    3061 gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta 
    3121 acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg 
    3181 cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt 
    3241 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 
    3301 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 
    3361 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 
    3421 agattgtact gagagtgcac catatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
    3481 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
    3541 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
    3601 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
    3661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
    3721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
    3781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
    3841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
    3901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
    3961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
    4021 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
    4081 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
    4141 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
    4201 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
    4261 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
    4321 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
    4381 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
    4441 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
    4501 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
    4561 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
    4621 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
    4681 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
    4741 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
    4801 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
    4861 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
    4921 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
    4981 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
    5041 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
    5101 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
    5161 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
    5221 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
    5281 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
    5341 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
    5401 tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata ttttgttaaa 
    5461 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 
    5521 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 
    5581 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 
    5641 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 
    5701 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 
    5761 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 
    5821 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 
    5881 gggcgcgtcc cattcgcca