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pET-32c(+) DNA

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At a Glance

pET-32c(+), Image made with PlasMapper

Type

Cloning, Expression[1]

Origin of Replication

pBR322, f1

Copy #
Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
Trx Tag
His Tag
S Tag
MCS
lacI coding sequence

Accession Number




Notes

pET-32c(+) is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag thioredoxin protein[1]. The map for pET-32c(+) is the same as pET-32a(+) except that pET-32c(+) is a 5901 bp plasmid; add 1bp to each site beyond BamHI at 198.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

  1. 1.0 1.1 LaVallie, ER et al. (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Biotechnology (N.Y.) 11 187-93 PubMed EcoliWiki page

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc 
     241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc 
     301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc 
     361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac 
     421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc 
     481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc 
     541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca 
     601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact 
     661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac 
     721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt 
     781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc 
     841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat 
     901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc 
     961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg 
    1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 
    1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg 
    1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc 
    1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt 
    1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa 
    1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag 
    1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact 
    1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc 
    1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga 
    1561 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt 
    1621 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg 
    1681 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag 
    1741 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat 
    1801 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat 
    1861 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc 
    1921 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt 
    1981 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa 
    2041 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 
    2101 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct 
    2161 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
    2221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc 
    2281 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct 
    2341 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca 
    2401 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc 
    2461 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg 
    2521 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg 
    2581 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 
    2641 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 
    2701 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 
    2761 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 
    2821 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 
    2881 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 
    2941 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 
    3001 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 
    3061 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 
    3121 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 
    3181 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 
    3241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 
    3301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 
    3361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 
    3421 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg 
    3481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
    3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
    3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
    3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
    3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
    3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
    3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
    3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
    3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
    4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
    4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
    4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
    4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
    4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
    4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
    4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
    4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
    4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
    4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
    4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
    4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
    4741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
    4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
    4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
    4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
    4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
    5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
    5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
    5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
    5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
    5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
    5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
    5401 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta 
    5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 
    5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 
    5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 
    5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 
    5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 
    5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 
    5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 
    5881 cagggcgcgt cccattcgcc a