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pET-33b(+)

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At a Glance

pET-33b(+) Image made with PlasMapper

Type

Expression

Origin of Replication

pBR322, f1

Copy #
Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
PKA site
MCS
His Tag
lacI coding sequence

Accession Number




Notes

The pET-33b(+) vector is derived from pET-28b(+) and carries a 15 bp sequence encoding the protein kinase A (PKA) site ArgArgAlaSerVal, located between the thrombin cleavage and Nde I sites[1]

History

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Sources

Novagen

References

  1. de Arruda, M. and Burgess, R.R. (1995) inNovations 4, 7-8.

Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 
     181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat 
     241 gaacagatgc acgacggctg ccgcgcggca ccaggccgct gctgtgatga tgatgatgat 
     301 ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc tagaggggaa 
     361 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct 
     421 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg 
     481 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga 
     541 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca 
     601 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 
     661 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa 
     721 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg 
     781 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag 
     841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg 
     901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc 
     961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa 
    1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg 
    1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc 
    1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct 
    1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt 
    1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt 
    1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg 
    1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc 
    1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt 
    1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc 
    1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac 
    1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa 
    1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg 
    1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct 
    1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 
    1861 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg 
    1921 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 
    1981 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 
    2041 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 
    2101 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 
    2161 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg 
    2221 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta 
    2281 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc 
    2341 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 
    2401 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 
    2461 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 
    2521 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 
    2581 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 
    2641 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 
    2701 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 
    2761 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 
    2821 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
    2881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
    2941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 
    3001 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 
    3061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 
    3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
    3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
    3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
    3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
    3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
    3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
    3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
    3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
    3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
    3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
    3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
    3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
    3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
    3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
    3961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 
    4021 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat 
    4081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat 
    4141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 
    4201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 
    4261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa 
    4321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 
    4381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 
    4441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 
    4501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 
    4561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 
    4621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 
    4681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 
    4741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 
    4801 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat 
    4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat 
    4921 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 
    4981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 
    5041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 
    5101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 
    5161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 
    5221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 
    5281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 
    5341 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca