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pET-39b(+)

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At a Glance

pET-39b(+) Image made with PlasMapper

Type

Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
DsbA Tag
His Tag
S Tag
MCS
lacI coding sequence

Accession Number




Notes

pET-39b(+) is designed for expression of DsbA fusion proteins.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 
     181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag 
     241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt 
     301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga 
     361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt tttctcgctt 
     421 aagtatttca ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata 
     481 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc 
     541 acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac 
     601 gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa 
     661 gcagaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct 
     721 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca 
     781 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca 
     841 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa 
     901 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca 
     961 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa 
    1021 atctttttca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat 
    1081 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga 
    1141 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 
    1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 
    1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 
    1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 
    1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc 
    1441 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 
    1501 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 
    1561 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 
    1621 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 
    1681 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 
    1741 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 
    1801 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 
    1861 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 
    1921 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 
    1981 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 
    2041 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 
    2101 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 
    2161 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 
    2221 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 
    2281 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa 
    2341 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 
    2401 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 
    2461 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 
    2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
    2581 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc 
    2641 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 
    2701 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 
    2761 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 
    2821 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc 
    2881 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt 
    2941 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 
    3001 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 
    3061 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 
    3121 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct 
    3181 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt 
    3241 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg 
    3301 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat 
    3361 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac 
    3421 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa 
    3481 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga 
    3541 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 
    3601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 
    3661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 
    3721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 
    3781 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 
    3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
    3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
    3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
    4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
    4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
    4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
    4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
    4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
    4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
    4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
    4441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
    4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
    4561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
    4621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
    4681 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 
    4741 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta 
    4801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg 
    4861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 
    4921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc 
    4981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg 
    5041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 
    5101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc 
    5161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 
    5221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 
    5281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 
    5341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 
    5401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 
    5461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 
    5521 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg 
    5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 
    5641 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 
    5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 
    5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 
    5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 
    5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 
    5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 
    6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 
    6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca