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pET-3c

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At a Glance

pET-3c,Image provided by Agilent-Genomics

Type

Cloning/Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent Genomics

Features

T7 Promoter
RBS
NdeI cloning site
T7 gene 10 leader
BamHI cloning site
T7 Terminator

Accession Number




Notes

Same features & purpose of pET-3a and b. Cloning & expression of recombinant proteins without any additional tags.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics
Novagen

References

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Sequence

THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.

   1  TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT  
  51  TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGGCTAGCA TGACTGGTGG  
 101  ACAGCAAATG GGTCGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA  
 151  GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT  
 201  CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA  
 251  TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC  
 301  CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT  
 351  GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG  
 401  CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA  
 451  AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG  
 501  GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG  
 551  TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT  
 601  TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT  
 651  CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT  
 701  AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT  
 751  TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC  
 801  CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC  
 851  ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT  
 901  TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT  
 951  GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC  
1001  TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA  
1051  GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA  
1101  ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC  
1151  CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC  
1201  AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC  
1251  AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG  
1301  ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA  
1351  CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT  
1401  GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT  
1451  TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG  
1501  GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG  
1551  TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC  
1601  GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA  
1651  AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT  
1701  GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT  
1751  TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC  
1801  AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA  
1851  AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT  
1901  GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT  
1951  CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA  
2001  GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA  
2051  AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA  
2101  GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG  
2151  ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT  
2201  GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA  
2251  CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA  
2301  CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC  
2351  TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC  
2401  CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT  
2451  ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT  
2501  GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT  
2551  AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC  
2601  GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC  
2651  GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT  
2701  GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA  
2751  CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA  
2801  ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA  
2851  CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC  
2901  CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG  
2951  TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT  
3001  GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT  
3051  TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA  
3101  TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC  
3151  GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG  
3201  GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG  
3251  GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG  
3301  CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG  
3351  ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT  
3401  GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG  
3451  CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC  
3501  CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG  
3551  GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA  
3601  CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC  
3651  GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG  
3701  TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA  
3751  CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC  
3801  CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA  
3851  GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA  
3901  AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA  
3951  CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG  
4001  CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG  
4051  GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC  
4101  GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG  
4151  CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG  
4201  AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA  
4251  GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA  
4301  AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC  
4351  TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT  
4401  GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA  
4451  GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC  
4501  ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT  
4551  ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC  
4601  GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT