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pET-3d

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At a Glance

pET-3d, Image provided by Agilent-Genomics

Type

Cloning/Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent Genomics

Features

T7 Promoter
RBS
NdeI cloning site
T7 gene 10 leader
BamHI cloning site
T7 Terminator

Accession Number




Notes

Same features & purpose of pET-3a and b and c. Cloning & expression of recombinant proteins without any additional tags.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent Genomics
Novagen

References

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Sequence

THE SEQUENCE PROVIDED IS NOT COMPATIBLE WITH RESTRICTION MAPS OR VECTOR INFORMATION OBTAINED PRIOR TO DECEMBER, 2001.

   1  TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT  
  51  TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTAGCAT GACTGGTGGA  
 101  CAGCAAATGG GTCGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG  
 151  TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC  
 201  TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT  
 251  ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC  
 301  AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG  
 351  CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC  
 401  AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA  
 451  GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG  
 501  TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT  
 551  GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT  
 601  ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC  
 651  GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA  
 701  GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT  
 751  TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC  
 801  TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA  
 851  TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT  
 901  TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG  
 951  GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT  
1001  TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG  
1051  TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA  
1101  CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC  
1151  AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA  
1201  GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA  
1251  ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA  
1301  TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC  
1351  CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG  
1401  CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT  
1451  AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG  
1501  CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT  
1551  GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG  
1601  TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA  
1651  ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG  
1701  TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT  
1751  TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA  
1801  AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA  
1851  AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG  
1901  CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC  
1951  AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG  
2001  ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA  
2051  GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG  
2101  TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA  
2151  CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG  
2201  CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC  
2251  AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC  
2301  AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT  
2351  TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC  
2401  TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA  
2451  TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG  
2501  GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA  
2551  ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG  
2601  ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG  
2651  GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG  
2701  CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC  
2751  TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA  
2801  CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC  
2851  AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC  
2901  GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT  
2951  CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG  
3001  AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT  
3051  AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT  
3101  TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG  
3151  ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG  
3201  TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG  
3251  GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC  
3301  CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA  
3351  CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG  
3401  TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC  
3451  TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC  
3501  TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG  
3551  ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC  
3601  GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG  
3651  CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT  
3701  GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC  
3751  GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC  
3801  AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG  
3851  CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA  
3901  GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC  
3951  GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC  
4001  CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG  
4051  CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG  
4101  GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC  
4151  AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA  
4201  AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG  
4251  AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA  
4301  GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT  
4351  TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG  
4401  AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG  
4451  TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA  
4501  TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA  
4551  TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG  
4601  TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT