PortEco logo

pET-40b(+)

From EcoliWiki
Jump to: navigation, search

At a Glance

pET-40b(+) Image made with PlasMapper

Type

Expression

Origin of Replication

pBR322, f1

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
lac Operator
DsbC Tag
His Tag
S Tag
MCS
lacI coding sequence

Accession Number




Notes

pET-40b(+) is designed for expression of DsbC fusion proteins.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

See Help:References for how to manage references in EcoliWiki.


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 
     181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag 
     241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt 
     301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga 
     361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt accgctggtc 
     421 attttttggt gttcgtcgag aaattctttc atctctttcg gcggctggta acccggaaca 
     481 agtgtgccat tgctcagcac aactgccgga gtaccgctaa cgccaagctg gacgccaagt 
     541 gcgtaatggt cggcaatatc cacgtcgcaa ctggctggtg cgacgctttt acctgccatc 
     601 acatcatcaa acgctttgtt tttatctttc gcacaccaga tagctttcat ttctttctct 
     661 gcatcgctgt ccagcccctg gcgcgggaaa gcaagataac gcacggtgat ccccagcgcg 
     721 ttgtagtctg ccatttgctc atgcagtttg tggcagtaac cacaggtaat atcagtaaac 
     781 acggtgatga cgtgtttttc ctgcggcgct ttataaacga tcatctcttt ttcaagcgca 
     841 ttcaactgct ttaacagcat cttattggtg acattgaccg gagccgtgcc actaacgtca 
     901 tacattggcc cctgaatgat atgtttacca tcatcggtga tgtacaacac gccgctgtta 
     961 gtcagaactg tcttcatgcc agctacaggc gcgggctgaa tatcgctgct tttgatgccc 
    1021 attttggcta acgtttgttg aattgccgcg tcatcagcct gagcaaagcc tgaaaacgcc 
    1081 gctaacaaag taaacaacat aaaacctttc ttcatatgta tatctccttc ttaaagttaa 
    1141 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta 
    1201 ttaatttcgc gggatcgaga tcgatctcga tcctctacgc cggacgcatc gtggccggca 
    1261 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag 
    1321 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc 
    1381 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg 
    1441 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag 
    1501 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag 
    1561 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc 
    1621 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 
    1681 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 
    1741 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 
    1801 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 
    1861 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 
    1921 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 
    1981 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 
    2041 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 
    2101 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 
    2161 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 
    2221 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 
    2281 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 
    2341 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggacatctcg 
    2401 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc 
    2461 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 
    2521 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 
    2581 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 
    2641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta 
    2701 gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag 
    2761 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 
    2821 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt 
    2881 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct 
    2941 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat 
    3001 cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct 
    3061 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg 
    3121 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt 
    3181 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat 
    3241 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg 
    3301 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt 
    3361 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat 
    3421 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc 
    3481 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac 
    3541 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga 
    3601 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga 
    3661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 
    3721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 
    3781 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 
    3841 gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa 
    3901 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
    3961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
    4021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
    4081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
    4141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
    4201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
    4261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
    4321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
    4381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
    4441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
    4501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
    4561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
    4621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
    4681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
    4741 aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta cataaacagt 
    4801 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta 
    4861 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa 
    4921 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa 
    4981 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg 
    5041 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg 
    5101 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat 
    5161 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct 
    5221 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga 
    5281 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt 
    5341 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact 
    5401 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt 
    5461 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 
    5521 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 
    5581 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt 
    5641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
    5701 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt 
    5761 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 
    5821 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 
    5881 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 
    5941 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 
    6001 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 
    6061 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 
    6121 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 
    6181 ccattcgcca