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pET-41c(+)

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At a Glance

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pET-41c(+), Image provided by Novagen

Type

Cloning, Expression

Origin of Replication

f1, pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
N Terminal GST Tag
C Terminal His Tag
S Tag lacI
MCS

Accession Number




Notes

pET-41c(+) has the popular GST fusion tag for enhanced production and solubility.

The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is included. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing. When cloning into the PshaI site,the vector encoded sequence can be removed completely followed by the cleaving of the GST fusion protein with Enterokinase.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 
     181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc 
     241 ggatccccga tatcgccatg ggactcttgt cgtcgtcatc accggagcca ccaccggtac 
     301 ccagatctgg gctgtccatg tgctggcgtt cgaatttagc agcagcggtt tctttcatac 
     361 caattgcagt actaccgcgt ggcaccagac ccgcggagtg atggtgatgg tgatgaccag 
     421 aaccactagt tgaaccatcc gattttggag gatggtcgcc accaccaaac gtggcttgcc 
     481 agccctgcaa aggccatgct atatacttgc tggatttcaa gtacttatca atttgtggga 
     541 tagcttcaat acgtttttta aaacaaacta attttgggaa cgcatccagg cacattgggt 
     601 ccatgtataa aacaacatca agagcgtcat acaacatgaa gtcaggatgg gttacatgat 
     661 caccatttaa atatgtttta tgacataaac gatcttcgaa cattttcagc atttcaggta 
     721 gcttgctaag aaaatcaact ttgagagttt caaagtcttt actatatgca attctcgaaa 
     781 caccgtatct aatatccaaa accgctcctt caagcattga aatctctgca cgctcttttg 
     841 gacaaccacc caacatgttg tgcttgtcag ctatataacg tatgatggcc atagactgtg 
     901 ttaatttaac atcaccatca atataataag gaagattggg aaactccaaa cccaattcaa 
     961 actttttgtt tcgccattta tcaccttcat cgcgctcata caaatgctct tcatattttt 
    1021 cttcaagata ttccaaaaga agtcgagtgg gttgcacaag gcccttaatt ttccaataac 
    1081 ctagtatagg ggacatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg 
    1141 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag 
    1201 atcgatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc 
    1261 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg 
    1321 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt 
    1381 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct 
    1441 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca 
    1501 ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat 
    1561 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct 
    1621 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg 
    1681 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac 
    1741 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc 
    1801 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg 
    1861 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg 
    1921 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg 
    1981 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca 
    2041 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg 
    2101 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc 
    2161 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac 
    2221 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg 
    2281 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg 
    2341 ccattaccga gtccgggctg cgcgttggtg cggacatctc ggtagtggga tacgacgata 
    2401 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc 
    2461 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca 
    2521 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa 
    2581 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 
    2641 tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg 
    2701 ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 
    2761 ggcatgacta gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc 
    2821 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc 
    2881 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt 
    2941 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc 
    3001 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga 
    3061 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc 
    3121 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc 
    3181 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag 
    3241 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag 
    3301 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac 
    3361 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 
    3421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 
    3481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 
    3541 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 
    3601 gagtgcacca tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 
    3661 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
    3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
    3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
    3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
    3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
    3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
    4021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
    4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
    4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
    4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
    4261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
    4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
    4381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
    4441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
    4501 gattttggtc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta 
    4561 tgagccatat tcaacgggaa acgtcttgct ctaggccgcg attaaattcc aacatggatg 
    4621 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 
    4681 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 
    4741 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc 
    4801 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 
    4861 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 
    4921 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 
    4981 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 
    5041 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 
    5101 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 
    5161 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 
    5221 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 
    5281 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 
    5341 tgcagtttca tttgatgctc gatgagtttt tctaagaatt aattcatgag cggatacata 
    5401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
    5461 ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 
    5521 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 
    5581 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 
    5641 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 
    5701 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 
    5761 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 
    5821 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 
    5881 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gcca