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pET-42c(+)

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At a Glance

pET-42c(+), Image provided by Novagen

Type

Cloning, Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
N Terminal GST Tag
C Terminal His Tag
S Tag
lacI
MCS

Accession Number




Notes

One of pET-42b(+)'s unique feature is the popular GST fusion tag for enhanced production and solubility.

pET-42b(+)is specially used for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing. When cloning into the PshaI site, the vector encoded sequence can be removed completely followed by the cleaving of the GST fusion protein with Factor Xa.

Note that although the vector map shows pET-42a(+), the map is still the same for pET-42c(+), with different expression sites.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 
      181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc 
      241 ggatccccga tatcgccatg gacccgcgtc cctcaatacc ggagccacca ccggtaccca 
      301 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccaa 
      361 ttgcagtact accgcgtggc accagacccg cggagtgatg gtgatggtga tgaccagaac 
      421 cactagttga accatccgat tttggaggat ggtcgccacc accaaacgtg gcttgccagc 
      481 cctgcaaagg ccatgctata tacttgctgg atttcaagta cttatcaatt tgtgggatag 
      541 cttcaatacg ttttttaaaa caaactaatt ttgggaacgc atccaggcac attgggtcca 
      601 tgtataaaac aacatcaaga gcgtcataca acatgaagtc aggatgggtt acatgatcac 
      661 catttaaata tgttttatga cataaacgat cttcgaacat tttcagcatt tcaggtagct 
      721 tgctaagaaa atcaactttg agagtttcaa agtctttact atatgcaatt ctcgaaacac 
      781 cgtatctaat atccaaaacc gctccttcaa gcattgaaat ctctgcacgc tcttttggac 
      841 aaccacccaa catgttgtgc ttgtcagcta tataacgtat gatggccata gactgtgtta 
      901 atttaacatc accatcaata taataaggaa gattgggaaa ctccaaaccc aattcaaact 
      961 ttttgtttcg ccatttatca ccttcatcgc gctcatacaa atgctcttca tatttttctt 
     1021 caagatattc caaaagaagt cgagtgggtt gcacaaggcc cttaattttc caataaccta 
     1081 gtatagggga catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga 
     1141 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc 
     1201 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt 
     1261 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct 
     1321 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg 
     1381 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact 
     1441 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca 
     1501 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca 
     1561 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 
     1621 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 
     1681 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 
     1741 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 
     1801 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 
     1861 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 
     1921 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 
     1981 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 
     2041 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 
     2101 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 
     2161 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 
     2221 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 
     2281 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 
     2341 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg 
     2401 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 
     2461 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 
     2521 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 
     2581 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 
     2641 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga 
     2701 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 
     2761 atgactagca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt 
     2821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa 
     2881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg 
     2941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc 
     3001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg 
     3061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt 
     3121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt 
     3181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa 
     3241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 
     3301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 
     3361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 
     3421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 
     3481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 
     3541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 
     3601 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 
     3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
     3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 
     4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     4501 tttggtcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga 
     4561 gccatattca acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg 
     4621 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 
     4681 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 
     4741 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 
     4801 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 
     4861 ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 
     4921 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 
     4981 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 
     5041 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 
     5101 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 
     5161 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 
     5221 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 
     5281 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 
     5341 agtttcattt gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt 
     5401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     5461 cctgaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 
     5521 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 
     5581 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 
     5641 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 
     5701 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 
     5761 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 
     5821 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 
     5881 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a 
[Category:lacI]]