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pET-43.1a(+)

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At a Glance

pET-43.1a(+), Image provided by Novagen

Type

Cloning and Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
N Terminal Nus Tag
C Terminal His Tag
S Tag
MCS
C Terminal HSV Tag
lacI

Accession Number




Notes

pET-43.1a(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. The f1 origin is oriented to ensure that that infection with helper phage produces virions containing single stranded DNA corresponding to the coding strand. When cloning into the PshA I or Sma I sites, the vector encoded sequence can be completely removed by cleavage of the Nus•Tag fusion protein with Enterokinase or Thrombin, respectively.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References

[1] [2] [3] [4] [5]

  1. Segers, K et al. (2007) Design of protein membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V. Proc. Natl. Acad. Sci. U.S.A. 104 12697-702 PubMed EcoliWiki page
  2. Carré-Mlouka, A et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. J. Biol. Chem. 281 24462-71 PubMed EcoliWiki page
  3. Nam, JO et al. (2005) Regulation of tumor angiogenesis by fastatin, the fourth FAS1 domain of betaig-h3, via alphavbeta3 integrin. Cancer Res. 65 4153-61 PubMed EcoliWiki page
  4. Goyer, A et al. (2004) Characterization and metabolic function of a peroxisomal sarcosine and pipecolate oxidase from Arabidopsis. J. Biol. Chem. 279 16947-53 PubMed EcoliWiki page
  5. Zheng, L et al. (2003) Production of a functional catalytic antibody ScFv-NusA fusion protein in bacterial cytoplasm. J. Biochem. 133 577-81 PubMed EcoliWiki page

Sequence

       1 tccggatata gttcctcctt tcagcaaaaa acccctcaag acccgtttag aggccccaag 
      61 gggttatgct agttattgct cagcggatac atatttgaat gtatttagaa aaataaacaa 
     121 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg 
     181 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt 
     241 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat 
     301 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc 
     361 ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc acttctgagt 
     421 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca 
     481 gcctaggaac gcccaactta attaacatta gtggtggtgg tggtggtgct cgagatcctc 
     541 tgggtcttca ggagcgagtt ctggctggct tgcacgtgta tacagctgtg cggccgcaag 
     601 cttcgaacgc gtatcgatgg taccgtcgac gtcctgcagg cgcgcctgta cagaattcgg 
     661 atccacgagc tcccggactc ttgtcgtcgt catcaatcgt accagaaccc gcgctgcccc 
     721 gggggaccag accagttggc ggtggcgagt ccatgtgctg gcgttcaaat ttcgcagcag 
     781 cggtttcttt acccgcggag tgatggtgat ggtgatgacc agaaccacta gtcgcttcgt 
     841 caccgaacca gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca 
     901 acccttcgat atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa 
     961 cgccacgggc ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat 
    1021 cgtcagccgg tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg 
    1081 catttttagc acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca 
    1141 acagctcttt catcggcaca taggccaatt cttccagcgt cgagaagcct tcttctacca 
    1201 gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt 
    1261 gcgcttccgc ctgatgctta gcttgcaggt cgtcaacggt catcacgttg agttcccaac 
    1321 cgctcagttg cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccagat 
    1381 taccggcttc aacggcgatg tccatggtgt gtttatcttc atccaccacg atagaagcaa 
    1441 cgtctgccgg tgccattgcg ttaatcacga actgcgccgg gttatcatcc cacaggacga 
    1501 tatcgatacg ctcgccaccc agttcagtag acaccgcctg aacacgcgcg ccacgcatac 
    1561 ctacgcaagc acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac 
    1621 gagaacccgg atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca 
    1681 cttcaatacg gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg 
    1741 cgccacgcgc ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga 
    1801 agttttcacg cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat 
    1861 ccagagagat gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt 
    1921 gttcacggaa ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa 
    1981 cctgttttgc cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa 
    2041 cgtaatcgcc caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct 
    2101 tggtcggctg ggtgacttca tcaacaacta accagcgacg gaaagtgtca aaatcaccgc 
    2161 ttttgcgatc gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg 
    2221 tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg 
    2281 atacggcttc aactacagcc aaaatttctt tgttcatatg tatatctcct tcttaaagtt 
    2341 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg 
    2401 tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca tcgtggccgg 
    2461 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga 
    2521 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg 
    2581 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc 
    2641 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg 
    2701 agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat 
    2761 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg 
    2821 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 
    2881 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 
    2941 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 
    3001 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 
    3061 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 
    3121 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 
    3181 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 
    3241 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 
    3301 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 
    3361 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 
    3421 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 
    3481 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 
    3541 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggacatct 
    3601 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 
    3661 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 
    3721 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 
    3781 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 
    3841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt 
    3901 tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc 
    3961 agctccttcc ggtgggcgcg gggcatgact agcatgatcg tgctcctgtc gttgaggacc 
    4021 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga 
    4081 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc 
    4141 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc 
    4201 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg 
    4261 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt 
    4321 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg 
    4381 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg 
    4441 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc 
    4501 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc 
    4561 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt 
    4621 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 
    4681 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 
    4741 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 
    4801 catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac cgcacagatg 
    4861 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 
    4921 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
    4981 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
    5041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
    5101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
    5161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
    5221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
    5281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
    5341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
    5401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
    5461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
    5521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
    5581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
    5641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
    5701 gaacgaaaac tcacgttaag ggattttggt catgacctat ttgtttattt ttctaaatac 
    5761 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa tcatgattga 
    5821 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 
    5881 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 
    5941 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 
    6001 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 
    6061 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 
    6121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 
    6181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 
    6241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 
    6301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 
    6361 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 
    6421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 
    6481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 
    6541 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 
    6601 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 
    6661 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 
    6721 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
    6781 gataatctca tgccatcgtg ccgccagaaa ttgtaaacgt taatattttg ttaaaattcg 
    6841 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 
    6901 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 
    6961 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 
    7021 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 
    7081 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 
    7141 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 
    7201 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 
    7261 cgtcccattc gccaa