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pET-43.1c(+)

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At a Glance

pET-43.1c(+), Image provided by Novagen

Type

Cloning, Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
N Terminal Nus Tag
C Terminal His Tag
S Tag
MCS
C Terminal HSV Tag
lacI

Accession Number




Notes

pET-43.1c(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 491 aa Nus•Tag™ protein. The T7 expression region is reversed on the circle map due to the sequence numbering by the pBR322 convention. The f1 origin is oriented to ensure that that infection with helper phage produces virions containing single stranded DNA corresponding to the coding strand. When cloning into the PshA I or Sma I sites, the vector encoded sequence can be removed completely by cleaving the Nus•Tag fusion protein with enterokinase or thrombin, respectively. Note that although the vector map shows pET-43.1a(+), the map is still the same for pET-43.1c(+), with different expression sites.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen

References


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca 
     121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat 
     181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt 
     241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 
     301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc 
     361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag 
     421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc 
     481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct 
     541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa 
     601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg 
     661 gatccacgag ctcggactct tgtcgtcgtc atcaatcgta ccagaacccg cgctgccccg 
     721 ggggaccaga ccagttggcg gtggcgagtc catgtgctgg cgttcaaatt tcgcagcagc 
     781 ggtttcttta cccgcggagt gatggtgatg gtgatgacca gaaccactag tcgcttcgtc 
     841 accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa 
     901 cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac 
     961 gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc 
    1021 gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc 
    1081 atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa 
    1141 cagctctttc atcggcacat aggccaattc ttccagcgtc gagaagcctt cttctaccag 
    1201 aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg 
    1261 cgcttccgcc tgatgcttag cttgcaggtc gtcaacggtc atcacgttga gttcccaacc 
    1321 gctcagttgc gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccagatt 
    1381 accggcttca acggcgatgt ccatggtgtg tttatcttca tccaccacga tagaagcaac 
    1441 gtctgccggt gccattgcgt taatcacgaa ctgcgccggg ttatcatccc acaggacgat 
    1501 atcgatacgc tcgccaccca gttcagtaga caccgcctga acacgcgcgc cacgcatacc 
    1561 tacgcaagca cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg 
    1621 agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttctggcac 
    1681 ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc 
    1741 gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc cagggcggaa 
    1801 gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc 
    1861 cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg 
    1921 ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac 
    1981 ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct gatcttcaac 
    2041 gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt 
    2101 ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaagtgtcaa aatcaccgct 
    2161 tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct ttgttgctgt 
    2221 cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga 
    2281 tacggcttca actacagcca aaatttcttt gttcatatgt atatctcctt cttaaagtta 
    2341 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt 
    2401 attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc 
    2461 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 
    2521 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 
    2581 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 
    2641 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 
    2701 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 
    2761 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 
    2821 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 
    2881 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 
    2941 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 
    3001 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 
    3061 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 
    3121 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 
    3181 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 
    3241 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 
    3301 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 
    3361 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 
    3421 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 
    3481 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 
    3541 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggacatctc 
    3601 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 
    3661 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 
    3721 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 
    3781 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
    3841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 
    3901 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 
    3961 gctccttccg gtgggcgcgg ggcatgacta gcatgatcgt gctcctgtcg ttgaggaccc 
    4021 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 
    4081 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 
    4141 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc 
    4201 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga 
    4261 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt 
    4321 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga 
    4381 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg 
    4441 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc 
    4501 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct 
    4561 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg 
    4621 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
    4681 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
    4741 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 
    4801 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc 
    4861 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 
    4921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
    4981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
    5041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
    5101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
    5161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
    5221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
    5281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
    5341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
    5401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
    5461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 
    5521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
    5581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
    5641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
    5701 aacgaaaact cacgttaagg gattttggtc atgacctatt tgtttatttt tctaaataca 
    5761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat catgattgaa 
    5821 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
    5881 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
    5941 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
    6001 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
    6061 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
    6121 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
    6181 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
    6241 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
    6301 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
    6361 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
    6421 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
    6481 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
    6541 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
    6601 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
    6661 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
    6721 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
    6781 ataatctcat gccatcgtgc cgccagaaat tgtaaacgtt aatattttgt taaaattcgc 
    6841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 
    6901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 
    6961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 
    7021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 
    7081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 
    7141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 
    7201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 
    7261 gtcccattcg cca