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pET-44 Ek/LIC

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At a Glance

pET-44 Ek/LIC, Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322, f1

Copy #

High

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
His Tag
Nus Tag
S Tag
HSV Tag
T7 Terminator
lacI Coding Sequence
MCS

Accession Number




Notes

The pET-44 Ek/LIC Vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal His•Tag®,Nus•Tag™ and S•Tag™ sequences. Inserts can cloned without the need for restriction digestion or ligation.

History

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Sources

Novagen

References

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Sequence

   1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
  61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca
 121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat 
 181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt
 241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
 301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc 
 361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag 
 421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc 
 481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct 
 541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa 
 601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg 
 661 gatccacgag ctcagatatc gttgaggaga agcccggact cttgtcgtcg tcatcaatcg 
 721 taccagaacc cgcgctgccc cgggggacca gaccagttgg cggtggcgag tccatgtgct 
 781 ggcgttcaaa tttcgcagca gcggtttctt tacccgcgga gtgatggtga tggtgatgac 
 841 cagaaccact agtcgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg 
 901 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg 
 961 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta 
1021 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg 
1081 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct 
1141 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccaat tcttccagcg 
1201 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg 
1261 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcttgcagg tcgtcaacgg 
1321 tcatcacgtt gagttcccaa ccgctcagtt gcgaagccag acgcacgttc tgaccgttac 
1381 ggccaatcgc ctgcgccaga ttaccggctt caacggcgat gtccatggtg tgtttatctt 
1441 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcacg aactgcgccg 
1501 ggttatcatc ccacaggacg atatcgatac gctcgccacc cagttcagta gacaccgcct 
1561 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg 
1621 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca 
1681 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg 
1741 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac 
1801 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg 
1861 cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc 
1921 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt 
1981 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa 
2041 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg 
2101 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac 
2161 ggaaagtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt 
2221 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc 
2281 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatac 
2341 tagaatggtg atggtggtga tggctgctgc ccatatgtat atctccttct taaagttaaa 
2401 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat 
2461 taatttcgcg ggatcgagat cgatctcgat cctctacgcc ggacgcatcg tggccggcat 
2521 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 
2581 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 
2641 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 
2701 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 
2761 gcgtcgagat cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc 
2821 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg 
2881 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 
2941 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 
3001 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 
3061 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 
3121 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 
3181 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 
3241 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 
3301 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 
3361 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 
3421 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 
3481 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 
3541 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 
3601 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg 
3661 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 
3721 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 
3781 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 
3841 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 
3901 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag 
3961 ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc 
4021 tccttccggt gggcgcgggg catgactagc atgatcgtgc tcctgtcgtt gaggacccgg 
4141 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt 
4201 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg 
4261 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag 
4321 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt 
4381 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc 
4441 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag 
4501 gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag 
4561 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt 
4621 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat 
4681 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 
4741 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 
4801 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat 
4861 cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt 
4921 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
4981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
5041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
5101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
5161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
5221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
5281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
5341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
5401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
5461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
5521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
5581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
5641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
5701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
5761 cgaaaactca cgttaaggga ttttggtcat gacctatttg tttatttttc taaatacatt 
5821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaatca tgattgaaaa 
5881 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
5941 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
6001 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
6061 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
6121 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
6181 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
6241 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
6301 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
6421 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
6481 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
6541 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
6601 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
6661 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
6721 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
6781 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
6841 aatctcatgc catcgtgccg ccagaaattg taaacgttaa tattttgtta aaattcgcgt 
6901 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 
6961 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 
7021 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 
7081 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 
7141 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 
7201 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 
7261 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 
7321 cccattcgcc a