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pET-44c(+)

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At a Glance

pET-44c(+), Image provided by Novagen

Type

Cloning and Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
N and C Terminal His Tag
S Tag
Nus Tag
MCS
C Terminal HSV Tag
lacI

Accession Number




Notes

The pET-44c(+) vector is specially designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag) protein. When E.coli cytoplasm is expressed as NusA fusions, the solubility of the various target protein in E.Coli cytoplasm is expressed. pET-43.1(+) vector series derived the pET-44(+) series which encodes an additional N-Terminal His•Tag sequence. When cloning into the PshA I or Sma I sites, the vector encoded sequence can be completely removed by the cleavage of the Nus•Tag fusion protein with enterokinase or thrombin, respectively. Note that although the vector map shows pET-44a(+), the map is still the same for pET-44c(+), with different expression sites.

History

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Sources

Novagen

References

See Help:References for how to manage references in EcoliWiki.


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca 
     121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat 
     181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt 
     241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 
     301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc 
     361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag 
     421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc 
     481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct 
     541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa 
     601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg 
     661 gatccacgag ctcggactct tgtcgtcgtc atcaatcgta ccagaacccg cgctgccccg 
     721 ggggaccaga ccagttggcg gtggcgagtc catgtgctgg cgttcaaatt tcgcagcagc 
     781 ggtttcttta cccgcggagt gatggtgatg gtgatgacca gaaccactag tcgcttcgtc 
     841 accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa 
     901 cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac 
     961 gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc 
    1021 gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc 
    1081 atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa 
    1141 cagctctttc atcggcacat aggccaattc ttccagcgtc gagaagcctt cttctaccag 
    1201 aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg 
    1261 cgcttccgcc tgatgcttag cttgcaggtc gtcaacggtc atcacgttga gttcccaacc 
    1321 gctcagttgc gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccagatt 
    1381 accggcttca acggcgatgt ccatggtgtg tttatcttca tccaccacga tagaagcaac 
    1441 gtctgccggt gccattgcgt taatcacgaa ctgcgccggg ttatcatccc acaggacgat 
    1501 atcgatacgc tcgccaccca gttcagtaga caccgcctga acacgcgcgc cacgcatacc 
    1561 tacgcaagca cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg 
    1621 agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttctggcac 
    1681 ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc 
    1741 gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc cagggcggaa 
    1801 gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc 
    1861 cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg 
    1921 ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac 
    1981 ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct gatcttcaac 
    2041 gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt 
    2101 ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaagtgtcaa aatcaccgct 
    2161 tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct ttgttgctgt 
    2221 cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga 
    2281 tacggcttca actacagcca aaatttcttt gttcatacta gaatggtgat ggtggtgatg 
    2341 gctgctgccc atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa 
    2401 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg 
    2461 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt 
    2521 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc 
    2581 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc 
    2641 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta 
    2701 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat 
    2761 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag 
    2821 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 
    2881 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 
    2941 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 
    3001 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 
    3061 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 
    3121 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 
    3181 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 
    3241 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 
    3301 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 
    3361 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 
    3421 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 
    3481 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 
    3541 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 
    3601 taccgagtcc gggctgcgcg ttggtgcgga catctcggta gtgggatacg acgataccga 
    3661 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 
    3721 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 
    3781 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 
    3841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 
    3901 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat 
    3961 ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 
    4021 tgactagcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta 
    4081 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa 
    4141 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg 
    4201 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct 
    4261 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga 
    4321 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta 
    4381 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta 
    4441 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa 
    4501 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac 
    4561 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg 
    4621 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 
    4681 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 
    4741 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 
    4801 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 
    4861 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 
    4921 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
    4981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
    5041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
    5101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
    5161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
    5221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
    5281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
    5341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
    5401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
    5461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
    5521 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
    5581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
    5641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
    5701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
    5761 ttggtcatga cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
    5821 acaataaccc tgataaatgc ttcaatcatg attgaaaaag gaagagtatg agtattcaac 
    5881 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 
    5941 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 
    6001 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 
    6061 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
    6121 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 
    6181 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 
    6241 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 
    6301 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 
    6361 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
    6421 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 
    6481 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 
    6541 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 
    6601 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 
    6661 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
    6721 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 
    6781 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgcca tcgtgccgcc 
    6841 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
    6901 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
    6961 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
    7021 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
    7081 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
    7141 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
    7201 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
    7261 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca