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pET-46 Ek/LIC

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At a Glance

pET-46 Ek/LIC, Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

pBR322, f1

Copy #

High

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 promoter
His Tag
Ek/LIC Cloning Site
S Tag
T7 Terminator
lacI Coding Sequence

Accession Number




Notes

The pET-46 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides. Inserts can be cloned without the need for restriction digestion and ligation.

History

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Sources

Novagen

References

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Sequence

   1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
  61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat 
 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg 
1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac 
1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 
2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 
2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac 
2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg 
2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 
2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 
2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg 
2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga 
2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg 
2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa 
2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt 
2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt 
3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct 
3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac 
3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag 
3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc 
3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc 
3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc 
3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt 
3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg 
3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc 
3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat 
3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt 
3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc 
3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc 
3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag 
3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt 
4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat 
4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag 
4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac 
4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg 
4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg 
4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt 
4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc 
4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact 
4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg 
4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga 
4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc 
4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt 
4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg 
4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc 
4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 
4921 aaataatttt gtttaacttt aagaaggaga tataccatgg cacatcacca ccaccatcac 
4981 gtggatgacg acgacaagag tccgggcttc tcctcaaatc tcgagtctgg taaagaaacc
5041 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc 
5101 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 
5161 ttgaggggtt ttttgctgaa aggaggaact atatccggat