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pET-48b(+)

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At a Glance

PET-48b(+).jpg Image provided by Novagen

Type

Cloning, Expression

Origin of Replication

f1
pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
His Tag
N Terminal Trx Tag
MCS
C Terminal S Tag
lacI

Accession Number




Notes

The pET-48b(+) vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein that can be cleaved with the human rhinovirus (HRV) 3C protease. This plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the rhinovirus 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites. There is also an optional C-terminal S•Tag™ coding sequence that is compatible with purification, detection, and quantification

History

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Sources

Novagen You can help EcoliWiki by writing about the available sources of this plasmid.

References

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Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
     121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc 
     181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct 
     241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc 
     301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga 
     361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta 
     421 gtatggccag aaccagaacc ggccaggtta gcgtcgagga actctttcaa ctgaccttta 
     481 gacagtgcac ccactttggt tgccgccact tcaccgtttt tgaacagcag cagagtcggg 
     541 ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt cagttttgca 
     601 acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc gatcattttg 
     661 cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc tttgagtaca 
     721 tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatatg tatatctcct 
     781 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta 
     841 tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac gccggacgca 
     901 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 
     961 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 
    1021 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 
    1081 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 
    1141 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt 
    1201 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg 
    1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 
    1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 
    1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 
    1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 
    1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 
    1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 
    1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 
    1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 
    1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 
    1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 
    1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 
    1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 
    1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 
    2041 gcggacatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 
    2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 
    2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 
    2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
    2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
    2341 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt 
    2401 caacccagtc agctccttcc ggtgggcgcg gggcatgact agcatgatcg tgctcctgtc 
    2461 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat 
    2521 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg 
    2581 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac 
    2641 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 
    2701 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca 
    2761 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac 
    2821 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc 
    2881 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt 
    2941 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca 
    3001 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc 
    3061 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
    3121 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
    3181 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 
    3241 aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg tgtgaaatac 
    3301 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 
    3361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
    3421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
    3481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
    3541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
    3601 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
    3661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
    3721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
    3781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
    3841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
    3901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
    3961 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
    4021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
    4081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
    4141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat aaaactgtct 
    4201 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc 
    4261 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 
    4321 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca 
    4381 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 
    4441 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 
    4501 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta 
    4561 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 
    4621 ttgcattcga ttcctgtttg taattgtcct tttaacagtg atcgcgtatt tcgtctcgct 
    4681 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 
    4741 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg 
    4801 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 
    4861 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 
    4921 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 
    4981 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 
    5041 ttctaagaat taattcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
    5101 aggggttccg cgcacatttc cccgaaaagt gccacctgaa attgtaaacg ttaatatttt 
    5161 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 
    5221 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 
    5281 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 
    5341 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 
    5401 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 
    5461 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 
    5521 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 
    5581 gctacagggc gcgtcccatt cgcca