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pET-9a

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At a Glance

pET-9a Image made with PlasMapper

Type

Cloning/Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
T7 Transcription start
T7•Tag
MCS
T7 Terminator

Accession Number




Notes

pET-9a[1][2][3] is a 4341bp vector for cloning and expression. pET vectors[4] pET-9a-d(+) possess an N-terminal T7•Tag and a MCS that includes a BamHI cloning site.

History

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Sources

Novagen
Promega

References

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See Help:References for how to manage references in EcoliWiki.

  1. Shen, R et al. (2004) Escherichia coli nucleoside diphosphate kinase interactions with T4 phage proteins of deoxyribonucleotide synthesis and possible regulatory functions. J. Biol. Chem. 279 32225-32 PubMed EcoliWiki page
  2. Dai, KS & Liew, CC (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. J. Biol. Chem. 276 23992-9 PubMed EcoliWiki page
  3. Hoffman, BJ et al. (1995) Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21 (DE3): process control yielding high levels of metal-incorporated, soluble protein. Protein Expr. Purif. 6 646-54 PubMed EcoliWiki page
  4. Hamburger, AE et al. (2005) Steric accessibility of the HIV-1 gp41 N-trimer region. J. Biol. Chem. 280 12567-72 PubMed EcoliWiki page

[1] [2] [3] [4]

Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
     241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
     301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
     361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
     421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
     481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc 
     541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac 
     601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct 
     661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 
     721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 
     781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 
     841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 
     901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag 
     961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 
    1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 
    1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 
    1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 
    1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 
    1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 
    1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 
    1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg 
    1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 
    1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag 
    1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg 
    1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc 
    1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat 
    1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa 
    1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg 
    1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc 
    1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 
    1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc 
    2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc 
    2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat 
    2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa 
    2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga 
    2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 
    2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
    2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
    2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 
    2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc 
    2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 
    2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
    2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
    2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
    2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
    2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
    2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
    3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
    3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
    3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
    3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
    3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
    3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
    3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
    3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca 
    3481 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 
    3541 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat 
    3601 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag 
    3661 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca 
    3721 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat 
    3781 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca 
    3841 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg 
    3901 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta 
    3961 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt 
    4021 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg 
    4081 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt 
    4141 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac 
    4201 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg 
    4261 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg 
    4321 ctcgatgagt ttttctaaga a


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