PortEco logo

pET-9b

From EcoliWiki
Jump to: navigation, search

At a Glance

pET-9b Image made with PlasMapper

Type

Cloning/Expression

Origin of Replication

pBR322


Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
T7 Transcription start
T7•Tag
MCS
T7 Terminator

Accession Number




Notes

pET-9b is the same as pET-9a with the exception that pET-9b is 1bp shorter at 4340bp; for the sites beyond BamH1 at 510 subtract 1bp.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen. Discontinued.
Promega. Discontinued.

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
     241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
     301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
     361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
     421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
     481 cagcttcctt tcgggctttg ttagcagccg gatcccgacc catttgctgt ccaccagtca 
     541 tgctagccat atgtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc 
     601 gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta 
     661 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 
     721 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 
     781 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 
     841 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 
     901 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt 
     961 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 
    1021 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg 
    1081 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc 
    1141 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat 
    1201 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg 
    1261 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt 
    1321 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct 
    1381 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc 
    1441 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt 
    1501 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc 
    1561 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg 
    1621 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc 
    1681 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc 
    1741 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat 
    1801 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga 
    1861 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca 
    1921 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga 
    1981 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc 
    2041 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca 
    2101 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg 
    2161 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac 
    2221 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac 
    2281 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc 
    2341 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 
    2401 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 
    2461 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 
    2521 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg 
    2581 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 
    2641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 
    2701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 
    2761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 
    2821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 
    2881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
    2941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
    3001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
    3061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
    3121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
    3181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
    3241 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
    3301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
    3361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
    3421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa 
    3481 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg 
    3541 aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 
    3601 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc 
    3661 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 
    3721 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 
    3781 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat 
    3841 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 
    3901 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 
    3961 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 
    4021 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc 
    4081 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 
    4141 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 
    4201 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 
    4261 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 
    4321 tcgatgagtt tttctaagaa