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pET-9d

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At a Glance

pET-9d Image made with PlasMapper

Type

Cloning/Expression

Origin of Replication

pBR322

Copy #

~40

Markers

Kanamycin

Link to Sequence

Novagen

Features

T7 Promoter
T7 Transcription start
T7•Tag
MCS
T7 Terminator

Accession Number




Notes

pET-9d[1] is the same as pET-9a with the exception that pET-9b is 3bp shorter at 4338bp and within the MCS there is an NcoI site in place of the NdeI site; for the sites beyond 551 in pET-3a subtract 3bp.

History

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Sources

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

  1. Eschenburg, S et al. (2005) A novel inhibitor that suspends the induced fit mechanism of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA). J. Biol. Chem. 280 14070-5 PubMed EcoliWiki page

[1]

Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
     241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
     301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
     361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
     421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
     481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat 
     541 gctagccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt 
     601 tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg 
     661 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 
     721 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 
     781 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 
     841 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 
     901 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca 
     961 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 
    1021 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 
    1081 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 
    1141 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 
    1201 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct 
    1261 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc 
    1321 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg 
    1381 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg 
    1441 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct 
    1501 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg 
    1561 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag 
    1621 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag 
    1681 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt 
    1741 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga 
    1801 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc 
    1861 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc 
    1921 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac 
    1981 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg 
    2041 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc 
    2101 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa 
    2161 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat 
    2221 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc 
    2281 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag 
    2341 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 
    2401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 
    2461 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 
    2521 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt 
    2581 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 
    2641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
    2701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
    2761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
    2821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
    2881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
    2941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
    3001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
    3061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
    3121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
    3181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
    3241 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
    3301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
    3361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
    3421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgaacaata 
    3481 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa 
    3541 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa 
    3601 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc 
    3661 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat 
    3721 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt 
    3781 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc 
    3841 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc 
    3901 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt 
    3961 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat 
    4021 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca 
    4081 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac 
    4141 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag 
    4201 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt 
    4261 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc 
    4321 gatgagtttt tctaagaa


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