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pGEX-5X-3

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At a Glance

pGEX-5X-3, Image made with PlasMapper

Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

tac Promoter
lac Operator
lac Iq
MCS
RBS

Accession Number

U13858



Notes

pGEX5X-3 is a derivative of pGEX-3X and possess a factor Xa recognition site. Requires very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional activity.

History

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Sources

GE Healthcare

References

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Sequence

       1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
      61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc caggaattcc cgggtcgact
     961 cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
    1021 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    1081 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    1141 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
    1201 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
    1261 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    1321 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    1381 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    1441 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    1501 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    1561 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    1621 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    1681 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    1741 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    1801 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    1861 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    1921 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    1981 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    2041 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    2101 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    2161 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    2221 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    2281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    2341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    2401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    2461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    2521 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
    2581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    2641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    2701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    2761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    2821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    2881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    2941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    3001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    3061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    3121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    3181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
    3241 ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
    3301 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
    3361 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
    3421 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
    3481 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
    3541 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
    3601 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
    3661 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
    3721 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
    3781 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
    3841 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
    3901 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
    3961 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
    4021 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
    4081 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
    4141 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
    4201 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
    4261 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
    4321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    4381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    4441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    4501 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
    4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    4681 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
    4741 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
    4801 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
    4861 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
    4921 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aatt