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pLysS

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At a Glance

pLysS Image made with PlasMapper

Type

Expression

Origin of Replication

p15A

Copy #
Markers

Chloramphenicol

Link to Sequence

Novagen

Features

T7 lysozyme coding seq. (pLysS)
T7 lysozyme coding seq. (pLysE)

Accession Number




Notes

This plasmid is not a cloning vector; it is used in lDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase.

History

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Sources

Novagen

References

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Sequence

      1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 
     51 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
    101 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC 
    151 CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 
    201 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT 
    251 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT 
    301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC 
    351 AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG 
    401 TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC 
    451 TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG 
    501 GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT 
    551 GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
    601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG 
    651 CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA 
    701 TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG 
    751 CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC 
    801 CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT 
    851 CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC 
    901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCTG 
    951 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC
   1001 ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT 
   1051 CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT 
   1101 CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
   1151 GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA 
   1201 GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC 
   1251 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT
   1301 TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG 
   1351 TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA 
   1401 TCTTATTAAT CAGATAAAAT ATTTCTAGAT TTCAGTGCAA TTTATCTCTT 
   1451 CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT GTAATTCTCA 
   1501 TGTTTGACAG CTTATCATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT 
   1551 TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC 
   1601 ATCGTCATCC TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT 
   1651 TATGCCGGTA CTGCCGGGCC TCTTGCGGGA TATCGTCCAT TCCGACAGCA 
   1701 TCGCCAGTCA CTATGGCGTG CTGCTAGCGC TATATGCGTT GATGCAATTT 
   1751 CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG GCCGCCGCCC 
   1801 AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG 
   1851 CGACCACACC CGTCCTGTGG ATCCGGCCCA TTGGCTGCCT CCCACACTTG 
   1901 GATATGCCTC CTCGGAGCCT TATAGAATTG TTTATAAGAC TTGCGCATTA 
   1951 TTTGACCTCC AATGCGAACA AAGGGAAACC GCTGTGGTCT CCCTTTAGTG 
   2001 AGTTCAATTA ATTATCCACG GTCAGAAGTG ACCAGTTCGT TCTTCTCCCA 
   2051 CCAACGCTTA AGGTCGAACG AAGGGCAAGC CTTCGGCGCC ACCTCATGAT 
   2101 GGGCGCGAAG ACCAGCGCCT TCGTACTTAG CCAGCAGTGT GACAAGCAGT 
   2151 GAGCGAAGGG ATTGCATTTG GGCTGGCGTA AAGTTAGCGT CGAACTTACC 
   2201 TTTATCGTCG ATACCACCAA CAAGGCAGAC GCCGATAGAG TTGTGGTTGT 
   2251 AACCCTTAGC GTGAGAGCCT ACAGCCATCT CATCTCGTCC TGCCTCCACA 
   2301 GTACCGTCTC GCTTGATGAT AAAGTGGTAT CCCACATCGA GCCAACCCTG 
   2351 CTCTTTGTGC CACTGGCGAA TCTCACGGAC ACCAACATTC TGACTTGGCT 
   2401 TGGTAGCCGA GCAGTGAACA AAGATTGCGT CAGTAGATTC ACGTTGTTTA 
   2451 AACTGTACAC GAGCCATTAT TTCTTTCCTC CTTTCCTTTT TAATCTATCA 
   2501 AAGGGGACCC GGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG 
   2551 CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG 
   2601 AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT 
   2651 ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA 
   2701 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG
   2751 GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG ACCGATGCCC 
   2801 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC 
   2851 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC 
   2901 AGGTGCCGGC AGCGCTCTGG GTCATTTTCG GCGAGGACCG CTTTCGCTGG 
   2951 AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA TCTTGCACGC 
   3001 CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA 
   3051 AGCAGGCCAT TATCGCCGGC ATGGCGGCCG ACGCGCTGGG CTACGTCTTG 
   3101 CTGGCGTTCG CGACGCGAGG CTGGATGGCC TTCCCCATTA TGATTCTTCT 
   3151 CGCTTCCGGC GGCATCGGGA TGCCCGCGTT GCAGGCCATG CTGTCCAGGC 
   3201 AGGTAGATGA CGACCATCAG GGACAGCTTC AAGGATCGCT CGCGGCTCTT 
   3251 ACCAGCCTAA CTTCGATCAC TGGACCGCTG ATCGTCACGG CGATTTATGC 
   3301 CGCCTCGGCG AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC 
   3351 TATACCTTGT CTGCCTCCCC GCGTTGCGTC GCGGTGCATG GAGCCGGGCC 
   3401 ACCTCGACCT GAATGGAAGC CGGCGGCACC TCGCTAACGG ATTCACCACT 
   3451 CCAAGAATTG GAGCCAATCA ATTCTTGCGG AGAACTGTGA ATGCGCAAAC 
   3501 CAACCCTTGG CAGAACATAT CCATCGCGTC CGCCATCTCC AGCAGCCGCA 
   3551 CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT GCGCATGATC 
   3601 GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG 
   3651 TTAGCAGAAT GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT 
   3701 GCAAAACGTC TGCGACCTGA GCAACAACAT GAATGGTCTT CGGTTTCCGT 
   3751 GTTTCGTAAA GTCTGGAAAC GCGGAAGTCC CCTACGTGCT GCTGAAGTTG 
   3801 CCCGCAACAG AGAGTGGAAC CAACCGGTGA TACCACGATA CTATGACTGA 
   3851 GAGTCAACGC CATGAGCGGC CTCATTTCTT ATTCTGAGTT ACAACAGTCC 
   3901 GCACCGCTGT CCGGTAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 
   3951 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC 
   4001 CGGCAGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG 
   4051 CCGAAACAAG CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG 
   4101 CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTAAC 
   4151 CGTTTTTATC AGGCTCTGGG AGGCAGAATA AATGATCATA TCGTCAATTA 
   4201 TTACCTCCAC GGGGAGAGCC TGAGCAAACT GGCCTCAGGC ATTTGAGAAG 
   4251 CACACGGTCA CACTGCTTCC GGTAGTCAAT AAACCGGTAA ACCAGCAATA 
   4301 GACATAAGCG GCTATTTAAC GACCCTGCCC TGAACCGACG ACCGGGTCGA 
   4351 ATTTGCTTTC GAATTTCTGC CATTCATCCG CTTATTATCA CTTATTCAGG 
   4401 CGTAGCACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG 
   4451 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
   4501 CCGACATGGA AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG 
   4551 CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG 
   4601 GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA 
   4651 CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT 
   4701 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 
   4751 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
   4801 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC 
   4851 CCATATCACC AGCTCACCGT CTTTCATTGC CATACG