pheT:Gene Product(s) - EcoliWiki
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pheT:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

Standard name

PheT

Synonyms

phenylalanine tRNA synthetase, beta subunit[1], B1713[2][1], PheT[2][1]

Product description

phenylalanyl-tRNA synthetase β-chain[2][3];

Component of ; phenylalanyl-tRNA synthetase[2][3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0005515

protein binding

PMID:15690043[4]

IPI: Inferred from Physical Interaction

UniProtKB:P08312

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005515

protein binding

PMID:16606699[6]

IPI: Inferred from Physical Interaction

UniProtKB:P0A7V0

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005624

membrane fraction

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc 12.5 [5]

complete

GO:0005737

cytoplasm

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc 12.5 [5]

complete

GO:0000049

tRNA binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002547
InterPro:IPR005121
SP_KW:KW-0820

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004532
InterPro:IPR009061
SP_KW:KW-0547

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0000287

magnesium ion binding

GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR005121
SP_KW:KW-0460
HAMAP:MF_00283

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0003723

RNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0004812

aminoacyl-tRNA ligase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0030

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0004826

phenylalanine-tRNA ligase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR004532
InterPro:IPR005121
EC:6.1.1.20
HAMAP:MF_00283

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR004532
InterPro:IPR005121
SP_KW:KW-0067
HAMAP:MF_00283

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0046872

metal ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0016874

ligase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0008033

tRNA processing

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005121

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0005737

cytoplasm

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004532
SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [5]

complete

GO:0006412

translation

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004532
SP_KW:KW-0648

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0006432

phenylalanyl-tRNA aminoacylation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR004532
InterPro:IPR005121
HAMAP:MF_00283

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0006418

tRNA aminoacylation for protein translation

P

Seeded from Riley et al 2006 [1].

required fields missing

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Interactions

Partner TypePartnerNotesReferences

Protein

Subunits of phenylalanyl-tRNA synthetase

could be indirect


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Notes

Localization

CompartmentDescriptionEvidenceNotes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc

MKFSELWLRE WVNPAIDSDA LANQITMAGL EVDGVEPVAG SFHGVVVGEV VECAQHPNAD
KLRVTKVNVG GDRLLDIVCG APNCRQGLRV AVATIGAVLP GDFKIKAAKL RGEPSEGMLC
SFSELGISDD HSGIIELPAD APIGTDIREY LKLDDNTIEI SVTPNRADCL GIIGVARDVA
VLNQLPLVQP EIVPVGATID DTLPITVEAP EACPRYLGRV VKGINVKAPT PLWMKEKLRR
CGIRSIDAVV DVTNYVLLEL GQPMHAFDKD RIEGGIVVRM AKEGETLVLL DGTEAKLNAD
TLVIADHNKA LAMGGIFGGE HSGVNDETQN VLLECAFFSP LSITGRARRH GLHTDASHRY
ERGVDPALQH KAMERATRLL IDICGGEAGP VIDITNEATL PKRATITLRR SKLDRLIGHH
IADEQVTDIL RRLGCEVTEG KDEWQAVAPS WRFDMEIEED LVEEVARVYG YNNIPDEPVQ
ASLIMGTHRE ADLSLKRVKT LLNDKGYQEV ITYSFVDPKV QQMIHPGVEA LLLPSPISVE
MSAMRLSLWT GLLATVVYNQ NRQQNRVRIF ESGLRFVPDT QAPLGIRQDL MLAGVICGNR
YEEHWNLAKE TVDFYDLKGD LESVLDLTGK LNEVEFRAEA NPALHPGQSA AIYLKGERIG
FVGVVHPELE RKLDLNGRTL VFELEWNKLA DRVVPQAREI SRFPANRRDI AVVVAENVPA
ADILSECKKV GVNQVVGVNL FDVYRGKGVA EGYKSLAISL ILQDTSRTLE EEEIAATVAK
CVEALKERFQ ASLRD
Mol. Wt

87.38 kDa (calc) [2]

pI
Extinction coefficient

64860 - 66485 (calc based on 14 Y, 8 W, and 13 C residues)

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Domains/Motifs/Modification Sites

TypeResiduesDescriptionNotes

Domain

39 - 148

tRNA-binding

UniProt:P07395

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Structure

Structures
  • Representative homolog:
    2RHQ|B (Staphylococcus haemolyticus)
    1-789 of 795 residues (E-value: 1.2e-123) (Percent Identity: 35.58)
  • View all other structures.
Models

View models at:

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Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:PHET-MONOMER

UniProt (EcoliWiki Page)

UniProt:P07395

PFAM (EcoliWiki Page)

PFAM:PF01588

RefSeq (EcoliWiki Page)

RefSeq:NP_416228

ModBase (EcoliWiki Page)

ModBase:P07395


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
  5. 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
  7. 7.0 7.1 Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page


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