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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

relA

Gene Synonym(s)

ECK2778, b2784, JW2755, RC[1]

Product Desc.

RELA[2][3]

ATP:GTP 3'-pyrophosphotransferase, ppGpp synthetase I; required for ppGpp synthesis during stringent response to amino acid starvation; self regulated[4]

Product Synonyms(s)

(p)ppGpp synthetase I/GTP pyrophosphokinase[1], B2784[2][1], RelA[2][1], ppGpp synthase I[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): relA-mazEF[2][3], relA-chpRA, chpA

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(relA)    Textpresso(relA)

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Notes

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RelA has two physically and functionally distinct domains, the N-terminal domain, amino acids 1 to 455, is responsible for (p)ppGpp synthesis, and the C-terminal domain, amino acids 456 to 744, is responsible for self regulation.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

relA

Mnemonic

Relaxed

Synonyms

ECK2778, b2784, JW2755, RC[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

62.71 minutes 

MG1655: 2911673..2909439
gbrowse_img.php?coord=2909439..2911673&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2802579..2800345
gbrowse_img.php?coord=2800345..2802579&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2795251..2797257
gbrowse_img.php?coord=2795251..2797257&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2912307..2910073
gbrowse_img.php?coord=2910073..2912307&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3005551..3001981
gbrowse_img.php?coord=3001981..3005551&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2798546..2798821
gbrowse_img.php?coord=2798546..2798821&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔrelA (Keio:JW2755)

deletion

deletion

PMID:16738554

Shigen
CGSC10159[5]

relA::Tn5KAN

PMID:12519969

ASAP

At position 195 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20926

relA1

PMID:2556396

CGSC:4549

relA2

CGSC:5370

relA0

CGSC:6052

relA41

CGSC:12141

relA42

CGSC:12143

ΔrelA782::kan

PMID:16738554

CGSC:102636


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2755

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGTTGCGGTAAGAAGTGCACA

Primer 2:CCACTCCCGTGCAACCGACGCGC

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[5]

est. P1 cotransduction: 42% [6]
Synonyms:cysC95::Tn10

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[5]

est. P1 cotransduction: 43% [6]
Synonyms:fuc-3072::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10835

EchoBASE (EcoliWiki Page)

EchoBASE:EB0828

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2784

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3347

EcoGene (EcoliWiki Page)

EcoGene:EG10835

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RELA

CGSC (EcoliWiki Page)

CGSC:306

ASAP (EcoliWikiPage)

ASAP:ABE-0009125


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RelA

Synonyms

(p)ppGpp synthetase I/GTP pyrophosphokinase[1], B2784[2][1], RelA[2][1], ppGpp synthase I[2][1]

Product description

RELA[2][3]

ATP:GTP 3'-pyrophosphotransferase, ppGpp synthetase I; required for ppGpp synthesis during stringent response to amino acid starvation; self regulated[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [7]

complete

GO:0016597

amino acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912

F

Seeded from EcoCyc [7]

complete

GO:0016301

kinase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc [7]

complete

GO:0015969

guanosine tetraphosphate metabolic process

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004811
InterPro:IPR007685

P

Seeded from EcoCyc [7]

complete

GO:0008728

GTP diphosphokinase activity

GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.6.5

F

Seeded from EcoCyc [7]

complete

GO:0008152

metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002912

P

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0015949

nucleobase, nucleoside and nucleotide interconversion

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009281

cytosolic ribosome (sensu Bacteria)

C

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

acnB

PMID:16606699

Experiment(s):EBI-1143946

Protein

sxy

PMID:16606699

Experiment(s):EBI-1143946

Protein

adhE

PMID:16606699

Experiment(s):EBI-1143946

Protein

rpsM

PMID:16606699

Experiment(s):EBI-1143946

Protein

ftnB

PMID:16606699

Experiment(s):EBI-1143946

Protein

rplD

PMID:16606699

Experiment(s):EBI-1143946

Protein

ais

PMID:16606699

Experiment(s):EBI-1143946

Protein

slyD

PMID:16606699

Experiment(s):EBI-1143946

Protein

rplS

PMID:16606699

Experiment(s):EBI-1143946

Protein

yehH

PMID:16606699

Experiment(s):EBI-1143946

Protein

rplB

PMID:16606699

Experiment(s):EBI-1143946

Protein

aceE

PMID:16606699

Experiment(s):EBI-1143946

Protein

speG

PMID:16606699

Experiment(s):EBI-1143946

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MVAVRSAHIN KAGEFDPEKW IASLGITSQK SCECLAETWA YCLQQTQGHP DASLLLWRGV
EMVEILSTLS MDIDTLRAAL LFPLADANVV SEDVLRESVG KSVVNLIHGV RDMAAIRQLK
ATHTDSVSSE QVDNVRRMLL AMVDDFRCVV IKLAERIAHL REVKDAPEDE RVLAAKECTN
IYAPLANRLG IGQLKWELED YCFRYLHPTE YKRIAKLLHE RRLDREHYIE EFVGHLRAEM
KAEGVKAEVY GRPKHIYSIW RKMQKKNLAF DELFDVRAVR IVAERLQDCY AALGIVHTHY
RHLPDEFDDY VANPKPNGYQ SIHTVVLGPG GKTVEIQIRT KQMHEDAELG VAAHWKYKEG
AAAGGARSGH EDRIAWLRKL IAWQEEMADS GEMLDEVRSQ VFDDRVYVFT PKGDVVDLPA
GSTPLDFAYH IHSDVGHRCI GAKIGGRIVP FTYQLQMGDQ IEIITQKQPN PSRDWLNPNL
GYVTTSRGRS KIHAWFRKQD RDKNILAGRQ ILDDELEHLG ISLKEAEKHL LPRYNFNDVD
ELLAAIGGGD IRLNQMVNFL QSQFNKPSAE EQDAAALKQL QQKSYTPQNR SKDNGRVVVE
GVGNLMHHIA RCCQPIPGDE IVGFITQGRG ISVHRADCEQ LAELRSHAPE RIVDAVWGES
YSAGYSLVVR VVANDRSGLL RDITTILANE KVNVLGVASR SDTKQQLATI DMTIEIYNLQ
VLGRVLGKLN QVPDVIDARR LHGS
Length

744

Mol. Wt

83.875 kDa

pI

6.8 (calculated)

Extinction coefficient

93,280 - 94,655 (calc based on 22 Y, 11 W, and 11 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:RELA-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AG20

PFAM (EcoliWiki Page)

PFAM:PF04607

RefSeq (EcoliWiki Page)

RefSeq:NP_417264


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

relA-mazEF

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2911653..2911693&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2784 (EcoliWiki Page)

NCBI GEO profiles for relA

microarray

GenExpDB:b2784 (EcoliWiki Page)

Summary of data for relA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (2911600..2911755) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:B4[8]

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Notes

Accessions Related to relA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10835

EchoBASE (EcoliWiki Page)

EchoBASE:EB0828

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2784

EcoGene (EcoliWiki Page)

EcoGene:EG10835


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Arabidopsis thaliana

  • AT4G02260 (score: 1.000; bootstrap: 73%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000024795 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00038968 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00014148 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000018036 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-050417-377 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-050417-378 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0230182 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0039650 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA11268-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000330721 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000006766 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000024331 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:1915945 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6ATB4 (score: 1.000; bootstrap: 97%)
  • Q9AYT4 (score: 0.998)
  • Q75IS2 (score: 0.549)
  • Q6ATB2 (score: 0.496)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000012759 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000016445 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000171044 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00034759001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

RELA

From SHIGELLACYC

E. coli O157

RELA

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF04607


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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