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rluA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RluA

Synonyms

pseudouridine synthase for 23S rRNA (position 746) and tRNAphe(position 32)[1], B0058[2][1], YabO[2][1], RluA[2][1]

Product description

23S rRNA and tRNA pseudouridine synthase[2][3]

Dual specificity tRNA and rRNA pseudouridine synthase; specific to 23S rRNA U746 and tRNAphe U32; non-essential gene[4]

EC number (for enzymes)

Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0016853

isomerase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0413

F

Seeded from EcoCyc [5]

complete

GO:0009982

pseudouridine synthase activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006145
InterPro:IPR006224

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0008033

tRNA processing

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0819

P

Seeded from EcoCyc [5]

complete

GO:0006364

rRNA processing

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0698

P

Seeded from EcoCyc [5]

complete

GO:0031119

tRNA pseudouridine synthesis

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [5]

complete

GO:0001522

pseudouridine synthesis

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006145
InterPro:IPR006224

P

Seeded from EcoCyc [5]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0000455

enzyme-directed rRNA pseudouridine synthesis

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [5]

complete

GO:0009451

RNA modification

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0031118

rRNA pseudouridine synthesis

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0031119

tRNA pseudouridine synthesis

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0009451

RNA modification

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0009982

pseudouridine synthase activity

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0001522

pseudouridine synthesis

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0008033

tRNA processing

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006364

rRNA processing

PMID:10383384[7]

IMP: Inferred from Mutant Phenotype

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc

MGMENYNPPQ EPWLVILYQD DHIMVVNKPS GLLSVPGRLE EHKDSVMTRI QRDYPQAESV
HRLDMATSGV IVVALTKAAE RELKRQFRER EPKKQYVARV WGHPSPAEGL VDLPLICDWP
NRPKQKVCYE TGKPAQTEYE VVEYAADNTA RVVLKPITGR SHQLRVHMLA LGHPILGDRF
YASPEARAMA PRLLLHAEML TITHPAYGNS MTFKAPADF
Length

219

Mol. Wt

24.86 kDa

pI

8.1 (calculated)

Extinction coefficient

29,910 - 30,160 (calc based on 9 Y, 3 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P0AA37



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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12609-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AA37

UniProt (EcoliWiki Page)

UniProt:P0AA37

PFAM (EcoliWiki Page)

PFAM:PF00849

RefSeq (EcoliWiki Page)

RefSeq:NP_414600


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 5.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  6. EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 Raychaudhuri S et al. (1999) Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA. J Biol Chem 274: 18880-6 PubMed EcoliWiki page

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