rluD:Gene Product(s) - EcoliWiki
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rluD:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

Standard name

RluD

Synonyms

23S rRNA pseudouridine synthase[1], B2594[2][1], YfiI[2][1], RluD[2][1], SfhB[2][1]

Product description

23S rRNA pseudouridine synthase[2][3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0005515

protein binding

PMID:15690043[4]

IPI: Inferred from Physical Interaction

UniProtKB:P0A8A4

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005515

protein binding

PMID:15690043[4]

IPI: Inferred from Physical Interaction

UniProtKB:P21165

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005515

protein binding

PMID:15690043[4]

IPI: Inferred from Physical Interaction

UniProtKB:Q9ZN60

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0009982

pseudouridine synthase activity

PMID:9843401[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0031118

rRNA pseudouridine synthesis

PMID:9814761[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0009982

pseudouridine synthase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006145
InterPro:IPR006224
InterPro:IPR006225

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0003723

RNA binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002942
InterPro:IPR006145
InterPro:IPR006224
InterPro:IPR006225
SP_KW:KW-0694

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009451

RNA modification

P

Seeded from EcoCyc 10.6[2].

required fields missing

GO:0016072

rRNA metabolic process

P

Seeded from EcoCyc 10.6[2].

required fields missing

GO:0031118

rRNA pseudouridine synthesis

PMID:9814761[7]

IMP

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009982

pseudouridine synthase activity

PMID:9843401[6]

IDA

F

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions

Partner TypePartnerNotesReferences
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Notes

Localization

CompartmentDescriptionEvidenceNotes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc

MAQRVQLTAT VSENQLGQRL DQALAEMFPD YSRSRIKEWI LDQRVLVNGK VCDKPKEKVL
GGEQVAINAE IEEEARFEPQ DIPLDIVYED EDIIIINKPR DLVVHPGAGN PDGTVLNALL
HYYPPIADVP RAGIVHRLDK DTTGLMVVAK TVPAQTRLVE SLQRREITRE YEAVAIGHMT
AGGTVDEPIS RHPTKRTHMA VHPMGKPAVT HYRIMEHFRV HTRLRLRLET GRTHQIRVHM
AHITHPLVGD PVYGGRPRPP KGASEAFIST LRKFDRQALH ATMLRLYHPI SGIEMEWHAP
IPQDMVELIE VMRADFEEHK DEVDWL
Mol. Wt

37.12 kDa (calc) [2]

pI
Extinction coefficient

28420 - 28545 (calc based on 8 Y, 3 W, and 1 C residues)

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Domains/Motifs/Modification Sites

TypeResiduesDescriptionNotes

Domain

18 - 91

S4 RNA-binding

UniProt:P33643

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Structure

Structures
  • Representative Escherichia coli structure:
    1V9F|A
    1-325 of 326 residues (E-value: 2.1e-176) (Percent Identity: 100.00)
  • View all other structures.
Models

View models at:

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Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12098-MONOMER

UniProt (EcoliWiki Page)

UniProt:P33643

PFAM (EcoliWiki Page)

PFAM:PF01479

RefSeq (EcoliWiki Page)

RefSeq:NP_417085

ModBase (EcoliWiki Page)

ModBase:P33643

PDB (EcoliWiki Page)

PDB:1V9F


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
  5. 5.0 5.1 5.2 5.3 5.4 5.5 5.6 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. 6.0 6.1 Huang L et al. (1998) Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA. Biochemistry 37: 15951-7 PubMed EcoliWiki page
  7. 7.0 7.1 Raychaudhuri S et al. (1998) A pseudouridine synthase required for the formation of two universally conserved pseudouridines in ribosomal RNA is essential for normal growth of Escherichia coli. RNA 4: 1407-17 PubMed EcoliWiki page


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