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rmf:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rmf

Mnemonic

Ribosome modulation factor

Synonyms

ECK0944, b0953, JW0936, ramB, rimF, rimG, res[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

21.88 minutes 

MG1655: 1014938..1015105
gbrowse_img.php?coord=1014938..1015105&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1032806..1032973
gbrowse_img.php?coord=1032806..1032973&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1016137..1016304
gbrowse_img.php?coord=1016137..1016304&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1068866..1069033
gbrowse_img.php?coord=1068866..1069033&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 917906..918073
gbrowse_img.php?coord=917906..918073&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Δrmf (Keio:JW0936)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8940[3]

rmf

PMID:12519969[4]

ASAP

At position 45 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20071

rimG21

CGSC:10347

rimG22

CGSC:11197

Δrmf-769::kan

PMID:16738554[2]

CGSC:102738


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0936

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAGAGACAAAAACGAGATCG

Primer 2:CCGGCCATTACTACCCTGTCCGC

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[3]

est. P1 cotransduction: 64% [6]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[3]

est. P1 cotransduction: 12% [6]

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG50004

EchoBASE (EcoliWiki Page)

EchoBASE:EB4298

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0953

EcoGene (EcoliWiki Page)

EcoGene:EG50004

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RMF

CGSC (EcoliWiki Page)

CGSC:31760

ASAP (EcoliWikiPage)

ASAP:ABE-0003227


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Glasner JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res 31: 147-51 PubMed EcoliWiki page
  5. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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