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rne:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

rne

Mnemonic
Synonyms

ECK1069, b1084, JW1071, ams, hmp1, rne_1, smbB[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

24.58 minutes 

MG1655: 1143590..1140405
gbrowse_img.php?coord=1139405..1144590&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1145944..1142759
gbrowse_img.php?coord=1141759..1146944&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

rne-3071(ts)

temperature sensitive

PMID:8320240[2] PMID:1846032[3]

CGSC:7537


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW1071

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:gccAAAAGAATGTTAATCAACGCAACTCAG

Primer 2:cctTCAACAGGTTGCGGACGCGCAGGAGCG

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[5]

est. P1 cotransduction: % [6]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[5]

est. P1 cotransduction: 59% [6]

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10859

EchoBASE (EcoliWiki Page)

EchoBASE:EB0852

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1084

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1353

EcoGene (EcoliWiki Page)

EcoGene:EG10859

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RNE

CGSC (EcoliWiki Page)

CGSC:269

ASAP (EcoliWikiPage)

ASAP:ABE-0003668


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. McDowall KJ et al. (1993) The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. J Bacteriol 175: 4245-9 PubMed EcoliWiki page
  3. Babitzke P & Kushner SR (1991) The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli. Proc Natl Acad Sci U S A 88: 1-5 PubMed EcoliWiki page
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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