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rne:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rne

Mnemonic

RNase E

Synonyms

ECK1069, b1084, JW1071, ams, hmp1, rne_1, smbB[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

24.58 minutes 

MG1655: 1143590..1140405
gbrowse_img.php?coord=1140405..1143590&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1158936..1155751
gbrowse_img.php?coord=1155751..1158936&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1145944..1142759
gbrowse_img.php?coord=1142759..1145944&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1199035..1195850
gbrowse_img.php?coord=1195850..1199035&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1044330..1047515
gbrowse_img.php?coord=1044330..1047515&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

rne-3071(ts)

temperature sensitive

PMID:8320240[2] PMID:1846032[3]

CGSC:7537


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1071

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:gccAAAAGAATGTTAATCAACGCAACTCAG

Primer 2:cctTCAACAGGTTGCGGACGCGCAGGAGCG

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[5]

est. P1 cotransduction: % [6]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[5]

est. P1 cotransduction: 59% [6]

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10859

EchoBASE (EcoliWiki Page)

EchoBASE:EB0852

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1084

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1353

EcoGene (EcoliWiki Page)

EcoGene:EG10859

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RNE

CGSC (EcoliWiki Page)

CGSC:269

ASAP (EcoliWikiPage)

ASAP:ABE-0003668


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. McDowall KJ et al. (1993) The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. J Bacteriol 175: 4245-9 PubMed EcoliWiki page
  3. Babitzke P & Kushner SR (1991) The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli. Proc Natl Acad Sci U S A 88: 1-5 PubMed EcoliWiki page
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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