rpsK:Gene Product(s) - EcoliWiki
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rpsK:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

Standard name

RpsK

Synonyms

30S ribosomal subunit protein S11[1], B3297[2][1], RpsK[2][1]

Product description

30S ribosomal subunit protein S11[2][3];

Component of ; 30S ribosomal subunit[2][3]; ribosome[2][3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0030529

ribonucleoprotein complex

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0687

C

Seeded from EcoCyc 12.5 [4]

complete

GO:0019843

rRNA binding

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0699
HAMAP:MF_01310

F

Seeded from EcoCyc 12.5 [4]

complete

GO:0006412

translation

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001971
HAMAP:MF_01310

P

Seeded from EcoCyc 12.5 [4]

complete

GO:0005840

ribosome

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001971

C

Seeded from EcoCyc 12.5 [4]

complete

GO:0003735

structural constituent of ribosome

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001971

F

Seeded from EcoCyc 12.5 [4]

complete

GO:0003723

RNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc 12.5 [4]

complete

GO:0022626

cytosolic ribosome

C

Seeded from EcoCyc 11.1[3].

required field missing

GO:0006412

translation

PMID:4579428[5]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 11.1[3].

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0022627

cytosolic small ribosomal subunit

PMID:4897206[6]

IDA: Inferred from Direct Assay

C

complete

GO:0070181

SSU rRNA binding

PMID:7585249[7]

IDA: Inferred from Direct Assay

F

S11 is a tertiary binding protein. It only binds to 16S rRNA in the presence of ribosomal proteins S4, S7, S8, S15, S17, S20, S16, S5, S12, and S6/S18.

complete

GO:0003735

structural constituent of ribosome

PMID:4897206[6]

IDA: Inferred from Direct Assay

F

S11 called protein 11 in this paper. See Wittmann et al. 1972 [8]'/> for correlation of 30S ribosomal proteins of E. coli isolated in different laboratories.

complete

GO:0070181

SSU rRNA binding

PMID:2505863[9]

IDA: Inferred from Direct Assay

F

S11 in the 30S subunit efficiently crosslinks to two regions of 16S rRNA: nt 649-734 and nt 735-823.

complete

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Interactions

Partner TypePartnerNotesReferences

Protein

Subunits of 30S ribosomal subunit

could be indirect

Protein

Subunits of ribosome

could be indirect

Protein

S7

Functional interaction inferred from similar effects of mutations in rpsK and rpsG on ribosome function.

PMID:12937172[10]

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Notes

Localization

CompartmentDescriptionEvidenceNotes
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Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc 

MAKAPIRARK RVRKQVSDGV AHIHASFNNT IVTITDRQGN ALGWATAGGS GFRGSRKSTP
FAAQVAAERC ADAVKEYGIK NLEVMVKGPG PGRESTIRAL NAAGFRITNI TDVTPIPHNG
CRPPKKRRV
Mol. Wt

13.84 kDa (calc) [2]

pI
Extinction coefficient

6990 - 7240 (calc based on 1 Y, 1 W, and 2 C residues)

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Domains/Motifs/Modification Sites

TypeResiduesDescriptionNotes

Modification Site

2

N-methylalanine

UniProt:P0A7R9

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Structure

Structures
  • Representative Escherichia coli structure:
    2GY9|K
    1-116 of 129 residues (E-value: 2.9e-60) (Percent Identity: 100.00)
  • Representative homolog:
    1I94|K (Thermus thermophilus)
    1-125 of 129 residues (E-value: 2.1e-34) (Percent Identity: 53.54)
  • View all other structures.
Models

View models at:

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Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10910-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0A7R9

PFAM (EcoliWiki Page)

PFAM:PF00411

RefSeq (EcoliWiki Page)

RefSeq:NP_417756


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  5. ↑ Held WA et al. (1973) Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components. J Biol Chem 248: 5720-30 PubMed EcoliWiki page
  6. ↑ 6.0 6.1 Hardy SJ et al. (1969) The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry 8: 2897-905 PubMed EcoliWiki page
  7. ↑ Powers T & Noller HF (1995) Hydroxyl radical footprinting of ribosomal proteins on 16S rRNA. RNA 1: 194-209 PubMed EcoliWiki page
  8. ↑ Wittmann HG et al. (1971) Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories. Mol Gen Genet 111: 327-33 PubMed EcoliWiki page
  9. ↑ Chiaruttini C et al. (1989) Crosslinking of ribosomal proteins S4, S5, S7, S8, S11, S12 and S18 to domains 1 and 2 of 16S rRNA in the Escherichia coli 30S particle. Biochimie 71: 839-52 PubMed EcoliWiki page
  10. ↑ Robert F & Brakier-Gingras L (2003) A functional interaction between ribosomal proteins S7 and S11 within the bacterial ribosome. J Biol Chem 278: 44913-20 PubMed EcoliWiki page


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