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rpsT:On One Page

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Standard Name

rpsT

Gene Synonym(s)

ECK0024, b0023, JW0022, sup(s20), supS20, supS[1][2]

Product Desc.

30S ribosomal subunit protein S20[2][3];

Component of 30S ribosomal subunit[2][3]; ribosome[2][3]

30S ribosomal subunit protein S20[4]

Product Synonyms(s)

30S ribosomal subunit protein S20[1], SupS[2][1], B0023[2][1], RpsT[2][1]

Function from GO

Biological Process

Molecular Function

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): rpsT[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(rpsT)    Textpresso(rpsT)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsT

Mnemonic

Ribosomal protein, small

Synonyms

ECK0024, b0023, JW0022, sup(s20), supS20, supS[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

0.45 minutes 

MG1655: 21078..20815
gbrowse_img.php?coord=20815..21078&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 25150..24887
gbrowse_img.php?coord=24887..25150&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 21078..20815
gbrowse_img.php?coord=20815..21078&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 21078..20815
gbrowse_img.php?coord=20815..21078&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 20815..21078
gbrowse_img.php?coord=20815..21078&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔrpsT (Keio:JW0022)

deletion

deletion

PMID:16738554

Shigen


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0022

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTAATATCAAATCAGCTAA

Primer 2:CCAGCCAGTTTGTTGATCTGTGC

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[5]

est. P1 cotransduction: 47% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 67% [6]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10919

EchoBASE (EcoliWiki Page)

EchoBASE:EB0912

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0023

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0027

EcoGene (EcoliWiki Page)

EcoGene:EG10919

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RPST

CGSC (EcoliWiki Page)

CGSC:212

ASAP (EcoliWikiPage)

ASAP:ABE-0000082


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RpsT

Synonyms

30S ribosomal subunit protein S20[1], SupS[2][1], B0023[2][1], RpsT[2][1]

Product description

30S ribosomal subunit protein S20[2][3];

Component of 30S ribosomal subunit[2][3]; ribosome[2][3]

30S ribosomal subunit protein S20[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0030529

ribonucleoprotein complex

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0687

C

Seeded from EcoCyc [7]

complete

GO:0019843

rRNA binding

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0699
HAMAP:MF_00500

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0006412

translation

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002583
HAMAP:MF_00500

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0005840

ribosome

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002583

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0003735

structural constituent of ribosome

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002583

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0003723

RNA binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002583
SP_KW:KW-0694

F

Seeded from EcoCyc [7]

complete

GO:0008073

ornithine decarboxylase inhibitor activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0022626

cytosolic ribosome

C

Seeded from EcoCyc 11.1[3].

required field missing

GO:0004857

enzyme inhibitor activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006412

translation

PMID:4579428

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 11.1[3].

Data in Table IV.

complete

GO:0003735

structural constituent of ribosome

PMID:4587209

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 11.1[3].

Data in Table 1.

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0022627

cytosolic small ribosomal subunit

PMID:4587209

IDA: Inferred from Direct Assay

C

Data in Figure 1.

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of 30S ribosomal subunit

could be indirect

Protein

Subunits of ribosome

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR
QAAKGLIHKN KAARHKANLT AQINKLA
Length

87

Mol. Wt

9.684 kDa

pI

11.6 (calculated)

Extinction coefficient

1,490 (calc based on 1 Y, W, and C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P0A7U7


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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10919-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0A7U7

PFAM (EcoliWiki Page)

PFAM:PF01649

RefSeq (EcoliWiki Page)

RefSeq:NP_414564


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

rpsT

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=21058..21098&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0023 (EcoliWiki Page)

NCBI GEO profiles for rpsT

microarray

GenExpDB:b0023 (EcoliWiki Page)

Summary of data for rpsT from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (20994..21229) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:H7[9]

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Notes

Accessions Related to rpsT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10919

EchoBASE (EcoliWiki Page)

EchoBASE:EB0912

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0023

EcoGene (EcoliWiki Page)

EcoGene:EG10919


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

RPST

From SHIGELLACYC

E. coli O157

RPST

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01649


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 8.2 8.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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